Transcriptome Analysis of Arabidopsis Root Development in Response to Treatment With Rhizobium sp. IRBG74
ABSTRACT: To examine how the Arabidopsis root development responds to the Rhizobium sp. IRBG74 treatment at the molecular level, we performed RNA-seq experiments. Our RNA-seq results suggest that expression of genes mainly involved in auxin signaling, cell wall and cell membrane integrity and transport is altered in response to colonization by Rhizobium sp. IRBG74. Overall design: Examination of Arabidopsis roots treated by Rhizobium sp. IRBG74 for 24 and 48 hours.
Rhizobium sp. IRBG74 not only nodulates Sesbania cannabina but also can enhance rice growth; however, the underlying molecular mechanisms are not clear. Here, we show that Rhizobium sp. IRBG74 colonizes the roots of Arabidopsis thaliana, which leads to inhibition in the growth of main root but enhancement in the formation of lateral roots. The promotion of lateral root formation by Rhizobium sp. IRBG74 in the fls2-1 mutant, which is insensitive to flagellin, is similar to the wild-type plant, wh ...[more]
Project description:Analysis of brassinosteroid (BR) and auxin effects on gene expression in Arabidopsis roots. Our genomic results indicate that BR and auxin induce largely opposite gene expression responses in primary roots. Overall design: RNA-Seq for 7-day-old Arabidopsis Col-0, dwf4, bri1-116, and bri1-116;bzr1-1D roots grown on regular medium and treated with brassinolide, auxin or mock solution for 4 hr.