Increasing miRNA sequencing accuracy using an RNA circularization approach

ABSTRACT: Accurate quantification of all microRNAs (miRNA) is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We have developed a new method of preparation of small RNA sequencing libraries (RealSeq®-AC) that ligates miRNAs with a single combo adapter and then circularizes the ligation products. When compared to other methods, RealSeq®-AC provided greatly reduced sequencing bias and allowed the identification of the largest variety of miRNAs in biological samples.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE107304 | GEO | 2018/07/01

REPOSITORIES: GEO