Gene expression profiling of human iPSC kidney organoid-derived epithelium

ABSTRACT: The kidney organoid differentiation protocol takes induced pluripotent stem cells through to kidney organoid via directed differentiation in approximately 25 days. The cells are grown in a monolayer in a dish for seven days and are subjected to growth factors before being pelleted on day seven. The organoids then continue to differentiate as a 3D structure, with at least 8 distinct kidney cell types identifiable around day 18. Here the EPCAM+ epithelial fraction was isolated from day 25 kidney organoids using MACS-enrichment and RNA-sequencing libraries generated.

ORGANISM(S): Homo sapiens

PROVIDER: GSE107305 | GEO | 2018/10/31

REPOSITORIES: GEO