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Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice

ABSTRACT: Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Keywords: knockout effect

ORGANISM(S): Mus musculus

PROVIDER: GSE10740 | GEO | 2008/03/06

SECONDARY ACCESSION(S): PRJNA107513

REPOSITORIES: GEO

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Transcription profiling of mouse colon from wild-type and Slc9a3 (NHE3)-deficient animals

Project description:Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Experiment Overall Design: Whole colon was dissected from 6-8 week old NHE3-deficient mice and their wild-type littermates and total RNA isolated for microarray analysis using Affymetrix murine MOE430 2.0 arrays

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