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Osteoprotection through the deletion of the transcription factor Rorβ in mice

ABSTRACT: Purpose: To identify gene expression changes (using RNAsequencing) in MC3T3-E1 cells where Rorβ has been deleted using CRISPR/Cas9 (and a control cell line) Method: MC3T3-Control and MC3T3-Rorβ-CRISPR(KO) cells were osteoblast differentiated for 2 days, and RNA was harvested (n=3 for each cell line) Results: We identified genes and pathways (using IPA) that were significantly altered in the Rorβ-CRISPR(KO) cell line, compared with the Control cell line Conclusions: Many pathways were changed, namely the Wnt pathway which is important to the biology of Rorβ and to osteoblast differentiation in general

ORGANISM(S): Mus musculus

PROVIDER: GSE107849 | GEO | 2017/12/09

SECONDARY ACCESSION(S): PRJNA421705

REPOSITORIES: GEO

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Increased osteoblast viability at alkaline pH in vitro provides a new perspective on bone regeneration

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