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RNAseq comparison of fadR deletion mutant and the wild type strain

ABSTRACT: To unravel the regulon of FadR (Saci_1107), comparative transcriptomic analysis was performed for the fadR deletion mutant versus the isogenic WT strain using RNA-seq approach.

ORGANISM(S): Sulfolobus acidocaldarius DSM 639

PROVIDER: GSE108018 | GEO | 2017/12/14

SECONDARY ACCESSION(S): PRJNA422245

REPOSITORIES: GEO

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RNA-seq comparison of fadR deletion mutant and the wild type strain

Project description:To unravel the regulon of FadR (Saci_1107) , comparative transcriptomic analysis was performed for the fadR deletion mutant versus the isogenic WT strain using RNA-seq approach.

2018-12-31 | E-MTAB-7584 | biostudies-arrayexpress

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Project description:To establish the SinR regulon in B. anthracis, gene expression of the fully virulent Ames strain and the isogenic sinR-null strains were compared using expression microarray analysis.

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Project description:The stress response of as sigZ deletion strain of B. cereus ATCC 14579 is monitored true time by use of microarrays. The sigZ regulon in ethanol stress response was determined and compared with the regulon determined by micorarray analysis of overexpression of sigZ. Keywords: stress response, comparative transcriptome study

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RNA deep sequencing data of the whole transcriptomes of the wild-type and crp2 (MSMEG_0539) mutant strains of Mycobacterium smegmatis MC2 155

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Monitoring the ethanol stress response of a sigM deletion strain of B. cereus ATCC 14579.

Project description:Here, the role of σM and its regulon in stress response and survival of B. cereus ATCC 14579 was assessed by comparative transciptome and phenotypic analysis of this strain and its sigM deletion strain. Exposure of B. cereus ATCC 14579 to a wide range of stresses revealed expression of sigM, encoding σM, to be up-regulated mainly in the presence of ethanol and after alkaline pH-shock. Next to this, disc diffusion tests showed the sigM deletion strain to be more sensitive to oxidizing agents and to be more resistant to cell-wall targeting antibiotics than the wild-type strain. The σM regulon was subsequently determined by comparative transcriptional analyses of the wild-type and its sigM-deletion strain after exposure to ethanol. The putative σM-regulon was shown to consist of 29 genes, several of these genes are predicted to be involved in counteracting oxidative stress, such as an NADH oxidase, a ferredoxin, and a lysine decarboxylase or could encode enzymes involved in methionine metabolism, leading toward L-cysteine production, including luxS. Screening of promoter upstream regions allowed for the assessment of a B. cereus consensus promoter binding site for σM. Since the consensus promoter binding site for B. cereus ATCC 14579 σM, its regulon and the predicted functionalities are different from the corresponding features in B. subtilis, it can be concluded that σM plays a unique role in B. cereus stress response and survival. Keywords: Stress response, comparative transcriptome study

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Expression analysis, A. actinomycetemcomitans wild type (JP2) vs CRP deletion mutant both grown anaerobically.

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2011-06-01 | GSE25459 | GEO

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Gene expression profiling of Staphylococcus aureus strain Newman and gene asp23 deletion mutant

Project description:RNA samples were extracted from both the WT and isogenic mutant NMΔasp23, respectively, and then subjected to RNA-seq analysis.

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