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LMP1 mediated gene expression changes in transfected CD10+ GC B cells

ABSTRACT: In this study, we have investigated the effect of LMP1 on gene expression in normal human GC B cells using a non-viral vector based system Keywords: transfection of viral oncogene in normal human B cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE10821 | GEO | 2008/10/10

SECONDARY ACCESSION(S): PRJNA107423

REPOSITORIES: GEO

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LMP1 mediated gene expression changes in transfected CD10+ GC B cells

Project description:In this study, we have investigated the effect of LMP1 on gene expression in normal human GC B cells using a non-viral vector based system Experiment Overall Design: Gene expression was compared between LMP1-transfected and control vector-transfected GC B cells from three patients. RNA from the FACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using significance analysis of microarrays (SAM) with a 1.5 fold change threshold and the q-value threshold set to 5%.

2008-10-26 | E-GEOD-10821 | biostudies-arrayexpress

LMP2A mediated gene expression changes in transfected CD10+ GCB cells

2013-04-19 | GSE46143 | GEO

KDM6B-induced gene expression changes in transfected CD10-positive GC B cells

2011-07-27 | GSE24044 | GEO

LMP2A mediated gene expression changes in transfected CD10+ GCB cells

Project description:In this study, we have investigated the effect of LMP2A on gene expression in normal human GC B cells using a non-viral vector based system Keywords: transfection of viral oncogene in normal human B cells Gene expression was compared between LMP2A-transfected and control vector-transfected GC B cells from three patients. RNA from the FACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed probe sets were identified using limma with a fold change >1.3 and p < 0.01

2013-04-19 | E-GEOD-46143 | biostudies-arrayexpress

KDM6B-induced gene expression changes in transfected CD10-positive GC B cells

Project description:In this study, we have investigated the effect of lysine-specific demethylase 6B (KDM6B) on gene expression in normal human germinal center (GC) B cells using a non-viral vector-based system. Gene expression was compared between KDM6B-transfected and control vector-transfected GC B cells from three patients. RNA from the MACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using Affymetrix GCOS pairwise analysis.

2011-07-26 | E-GEOD-24044 | biostudies-arrayexpress

Down-regulation of BLIMP1-alpha by the EBV oncogene, LMP1, disrupts the plasma cell differentiation program and prevents viral replication in B cells

Project description:In this study, we have investigated the effect of BLIMP1α on gene expression, cell differentiation and pathogenesis in normal human GC B cells using a non-viral vector based system Gene expression was compared among BLIMP1α, LMP1-transfected and control vector-transfected GC B cells from two patients. RNA from the FACS-sorted transfected GC B cells was amplified. Fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using the GCOS pair-wise analysis.

2011-07-26 | E-GEOD-27670 | biostudies-arrayexpress

Down-regulation of BLIMP1-alpha by the EBV oncogene, LMP1, disrupts the plasma cell differentiation program and prevents viral replication in B cells

2011-07-27 | GSE27670 | GEO

Expression profiling of HONE1 cell line stably transfected with LMP1

Project description:Using gene expression profiling technology from Capitalbio to find out the whole genome influence after LMP1 stably transfectted to HONE1 cell line. We analyzed gene expression change by LMP1 in HONE1 cell to find the active pathway and related biology function.

2010-06-20 | E-GEOD-18867 | biostudies-arrayexpress

Expression profiling of HONE1 cell line stably transfected with LMP1

Project description:Using gene expression profiling technology from Capitalbio to find out the whole genome influence after LMP1 stably transfectted to HONE1 cell line.

2009-11-05 | GSE18867 | GEO

Transcriptome analysis for LMP1 depletion rescue with Wildtype LMP1 or individual TES mutant LMP1 in GM12878 Lymphoblastoid cell line (LCL)

Project description:RNA-seq was used to characterize the LMP1 mediated regulation of host target gene regulation. Here, we stably expressed doxycycline-induced HA-tagged wildtype (WT) LMP1 or TES1 mutant (TES1m) oe TES2 mutant(TES2m) in GM12878 LCL. We then depleted LMP1 from these cells using CRISPR-Cas9 approach and treated the cells with 400ng/ml of doxycycline to induce expression of WT, TES1m or TES2m LMP1 to rescue from the LMP1 depletion mediated effects (including loss of cell viability) to understand and differenciate the role of LMP1 TES1 vs TES2 domains in LCL.

2023-08-25 | GSE240732 | GEO

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