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Allele-specific mRNA expression experiment in lactis-wickerhamii and lactis-marxianus interespecies hybrids

ABSTRACT: This experiment was performed to measure differences in pathway-level evolution of cis-regulation between closely related Kluyveromyces species. Sequencing of mRNA as well as DNA was performed in hybrids of Kl. lactis with two other species, Kl. wickerhamii and Kl. marxianus. Cells were grown overnight for DNA sequencing and for 4-8h to an OD600=0.7-1.0 for mRNA sequencing. DNA and mRNA reads were mapped to hybrid genomes, multimapping reads discarded, and allele-specific expression ratio for each gene was calculated after first normalizing mRNA reads to the number of DNA reads for each gene in each species. We found that the ribosomal protein genes showed pathway-level allele-specific expression differences between species in both interspecies hybrids.

ORGANISM(S): Kluyveromyces marxianus Kluyveromyces lactis x Kluyveromyces marxianus Kluyveromyces lactis x Kluyveromyces wickerhamii Kluyveromyces wickerhamii Kluyveromyces lactis

PROVIDER: GSE108389 | GEO | 2017/12/22

SECONDARY ACCESSION(S): PRJNA427120

REPOSITORIES: GEO

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Allele-specific mRNA expression experiment in lactis-wickerhamii and lactis-marxianus interespecies hybrids

Project description:Allele-specific mRNA expression experiment in lactis-wickerhamii and lactis-marxianus interespecies hybrids

| PRJNA427120 | ENA

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Project description:Ribosome profiling performed on interspecific hybrids of Sacharromyces cerevisiae and S. paradoxus in order to identify allele-specific expression indicative of cis-regulatory divergence at the level of mRNA abundance and protein translation. Two biological replicate libraries sequenced from ribosome protected fragments as well as poly-A-selected mRNA of hybrids in addition to one biological replicate library of poly-A selected mRNA from each parental strain.

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Allele-specific expression between Arabidopsis genus

2011-07-27 | GSE29037 | GEO

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