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Transcriptomics study of three Blumeria graminis formae speciales

ABSTRACT: We analyzed the gene expression and especially the expression of effector candidate genes in wheat and rye powdery mildews.

ORGANISM(S): Triticum aestivum Secale cereale Blumeria graminis f. sp. secalis Blumeria graminis f. sp. tritici x Triticosecale Blumeria graminis f. sp. triticale

PROVIDER: GSE108405 | GEO | 2018/01/10

SECONDARY ACCESSION(S): PRJNA427159

REPOSITORIES: GEO

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Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)

2012-03-27 | GSE30942 | GEO

Blumeria graminis

Project description:Pathogenic fungus that causes powdery mildew on grasses

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Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)

Project description:Powdery mildew is a very common plant disease and only few plants are immune. Host interactions have been identified and characterized for the pathosystems barley-B. graminis f. sp. tritici (Bgt) and wheat-B. graminis f. sp. hordei (Bgh), whereas no data are reported about powdery mildew and nonhost plants, such as rice. On the other hand rice nonhost resistance is widely unexploited and only few expression data are available. To characterize rice response during nonhost interaction with Bgh, a global expression analysis was performed by using the GeneChipM-BM-. Rice Genome Array. To describe rice gene expression profiles during nonhost interaction, 2 week-old rice plantlets were inoculated with Bgh. Treated (inoculated) and control (mock) samples were collected 24 hours post-inoculation for GeneChipM-BM-. Rice Genome Array hybridization. For transcript proM-oM-,M-^Aling experiments with powdery mildew, entire leaves were sampled from rice plantlets 2 weeks old (cv. Nipponbare). Treated and control (mock) rice leaves were collected 24 hours post inoculation. Three biological replicates for inoculated and control plants were extracted and analysed independently with the GeneChipM-BM-. Rice Genome Array.

2012-03-26 | E-GEOD-30942 | biostudies-arrayexpress

Transcriptomic analysis of Blumeria graminis triticale on different hosts

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Gene expression in Blumeria graminis f. sp. hordeii (Bgh) isolate DH14 during plant pathogenesis

Project description:We performed RNA-sequencing of Bgh-infected barley leaves at two different time-points after infection to examine gene expression in the barley powdery mildew isolate DH14 during plant pathogenesis.

2018-05-30 | GSE106282 | GEO

Transcriptomics study of three Blumeria graminis formae speciales

Project description:Transcriptomics study of three Blumeria graminis formae speciales

| PRJNA427159 | ENA

Transcriptomic analysis of Blumeria graminis triticale on different hosts

Project description:With this experiment we aimed do identify eventual genes that are differentially expressed by the fungal pathogen Blumeria graminis triticale when it grows on two different hosts (wheat and triticale) We used to fungal isolates, for each of them we infected wheat and triticale and we extracted RNA (and sequenced) from the infected plant tissue. Three technical replicates for each combinations plant-pathogen were used

2015-11-11 | E-GEOD-73399 | biostudies-arrayexpress

LC-MSMS of Hordeum vulgare epidermis containing haustoria from Blumeria graminis f.sp. hordei

Project description:Blumeria graminis f.sp. hordei is an obligate biotrohic fungal pathogen causing powdery mildew in barley. As for other biotrophic fungi, haustorial structures are at the centre of the biotrophic interaction and molecular exchanges, delivering fungal effectors or virulence factors, and taking nutrient from the host. Haustoria are originiated by the fungus, following successful penetration of the initial penetration peg through the plant cell call. Haustorial structures mainly of fungal origin, but they are surrounding by a plant component, the extrauhaustorial membrane and matrix (EHM and EHMx) forming the extrahuastorial complex (EHMc). The plant protein make-up of the plant extrahaustorial components remained unexplored, and this is a first study trying to describe plant proteome associated with haustoria using samples enriched for these structures. Therefore, proteomes of haustoria enriched samples from the epidermis of barley leaves infected with Blumeria graminins f.sp. hordei, the causing agent of barley powdery mildew, were compared to infected epidermis and un-infected epidermis to identify haustoria associated plant proteins. Haustoria were enriched from infected epidermis by digesting epidermal cell walls with cell wall degrading enzymes prior to enrichment for haustorial structures. Proteins identified in these samples were compared to infected and uninfected epidermis samples using a non-targeted label free semi-quantitation method.

2019-11-01 | PXD012684 | Pride

Expression analysis of individual homoeologous wheat genome- and rye genome-specific transcripts in a 2BS.2RL wheat-rye translocation

Project description:To distinguish transcripts expressed from each of the three wheat genomes and those from the rye chromatins, genomic probes generated from diploid progenitors of wheat and rye were synthesized

2015-02-24 | GSE58678 | GEO

Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)

Project description:Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012). B. graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to address the temporal regulation of membrane trafficking associated gene expression in barley-powdery mildew interactions. We created an isogenic panel of immune signaling mutants to address three main questions: (i) which Blumeria secreted proteins are differentially regulated in response to different compromised genotypes, (ii) which barley membrane trafficking genes are altered in response to pathogen attack, and (iii) how are these genes interacting across genotypes and infection stages.

2018-05-29 | GSE101304 | GEO

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