Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tissue-specific gene expression changes in l(3)mbt mutant ovaries [germline]

ABSTRACT: We performed RNAseq on embryos laid by somatically complemented l(3)mbt mutant germline to gain a genome-wide view of tissue-specific gene expression changes in L(3)mbt-depleted germline.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE108471 | GEO | 2018/02/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Tissue-specific gene expression changes in l(3)mbt mutant ovaries [soma]

Project description:We performed RNAseq on l(3)mbt mutant somatic ovaries to gain a genome-wide view of tissue-specific gene expression changes in L(3)mbt-depleted somatic ovaries.

2018-02-01 | GSE108472 | GEO

Tissue-specific gene expression changes in l(3)mbt mutant ovaries [germline]

Project description:Tissue-specific gene expression changes in l(3)mbt mutant ovaries [germline]

| PRJNA427342 | ENA

shotgun LC/MS/MS of L(3)mbt-IP samples using OSCs

Project description:L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed shotgun LC/MS/MS of L(3)mbt-IP samples and identified a new interactor in OSCs.

2022-08-15 | PXD026945 | Pride

Transcription profiling of mouse fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5

Project description:Gene expression in the fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5 were analyzed using Affymetrix Genechip to assess the effect of the gene knock-out on hematopoietic cells.

2004-11-30 | E-MEXP-219 | biostudies-arrayexpress

The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

Project description:In Drosophila, defects in asymmetric cell division can result in the formation of stem cell derived tumors. Here, we reveal a different mechanism that can result in the formation of very similar terminal brain tumor phenotypes. We demonstrate that brain tumors in l(3)mbt mutants originate from overproliferation of neuroepithelial cells in the optic lobes caused by de-repression of target genes in the Salvador-Warts-Hippo (SWH) pathway. We use ChIP-seq to identify L(3)mbt-binding sites and show that L(3)mbt binds to chromatin insulator elements. Mutating l(3)mbt or inhibiting the insulator protein mod(mdg4) results in upregulation of SWH pathway reporters. As l(3)mbt tumors are rescued by mutations in bantam or yorkie or by overexpression of expanded the deregulation of SWH pathway target genes is an essential step in brain tumor formation. Our data reveal that very different primary defects can result in the formation of brain tumors, which behave quite similarly in their advanced stages.

2011-08-29 | GSE29206 | GEO

Genome wide gene expression profiles of Drosophila l(3)mbt larval brains and cultured tumors

Project description:Mutants in the Drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. This data shows the changes in gene expression profile associated to mutations in l(3)mbt, both in situ in third instar larval brains and in tumors cultured for 1 5 and 10 (T1, T5, T10) rounds of allograft culture We performed genome-wide gene expression profiling of l(3)mbt[E2] and l(3)mbt[ts1] homozygous and transheterozygous larval brains raised at restrictive temperatures (29º C). We obtained three replicates for l(3)mbt[E2] at 17ªC (MBT_E2_17), l(3)mbt[ts1] at 17ºC (MBT_TS1_17), l(3)mbt[E2] at 29ºC (MBT_E2_29) and the transheterozygous at 29ºC (MBT_TS1_ME2), and six replicates l(3)mbt[ts1] at 29ºC (MBT_TS1_29). We also obtained three replicates for l(3)mbt[ts1] tumors at the first (T1), fifth (T5) and tenth (T10) round of allograft culture in adult flies, and six replicates for wild type w[1118] flies (WT).

2011-02-01 | E-GEOD-24917 | biostudies-arrayexpress

Tissue-specific gene expression changes in l(3)mbt mutant ovaries

Project description:Tissue-specific gene expression changes in l(3)mbt mutant ovaries

| PRJNA427340 | ENA

The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

2011-08-29 | E-GEOD-29206 | biostudies-arrayexpress

Transcriptome of yw fly, L(3)mbt mutant fly and Lint-O mutant fly were performed in triplicate in Drosophila melanogaster

Project description:L(3)mbt regulates the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed ChIP-seq of L(3)mbt and a newly identified interactor in OSCs to elucidate the genome-wide localization. Then we performed RNA-seq of OSCs before and after RNAi treatment of L(3)mbt and its interactors and performed differential expression analysis.

2022-08-14 | GSE205541 | GEO

Tissue-specific gene expression changes in l(3)mbt mutant ovaries [soma]

Project description:Tissue-specific gene expression changes in l(3)mbt mutant ovaries [soma]

| PRJNA427341 | ENA