Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In vitro modeling of human germ cell development using pluripotent stem cells

ABSTRACT: In this study, we developed a feeder-, serum-, and animal product-free culture condition, and successfully induced high percentage of SLCs from human PSCs. Genome-wide expression analyses demonstrated a significant upregulation of germ cell specific genes in SLCs derived from PSCs compared to in vivo isolated human CD90+ SSCs. And furthermore, under this optimized feeder-free condition SLCs have went through meiosis and formed putative round spermatids. Our data demonstrated a robust feeder-, serum- and xeno-free protocol to induce differentiation of PSCs to SLCs from which putative haploid spermatids may develop.

ORGANISM(S): Homo sapiens

PROVIDER: GSE108496 | GEO | 2018/12/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Generation of anti-GD2 CAR macrophages from human pluripotent stem cells for cancer immunotherapies

Project description:We generate macrophages from pluripotent stem cells (PSCs) with GD2 CARs integrated into AAVS1 locus. To produce CAR macrophages (CAR-M) we established a serum- and feeder-free differentiation protocol which generates macrophages through arterialized hemogenic endothelium.

2022-12-31 | GSE201174 | GEO

Extracellular matrix-dependent generation of integration- and xeno-free iPS cells using a modified mRNA transfection method

Project description:In this study, we sought to examine whether an extracellular matrix (ECM)-based xeno-free culture system that we recently established could be used together with a microRNA-enhanced mRNA reprogramming method for the generation of clinically safe iPS cells. The notable features of this method are (1) the use of a xeno-free/feeder-free culture system for the generation and expansion of iPS cells rather than the conventional labor-intensive culture systems using human feeder cells or human feeder-conditioned medium and (2) the enhancement of mRNA-mediated reprogramming via the delivery of microRNAs. Strikingly, we observed the early appearance of iPS cell colonies (~11 days), substantial reprogramming efficiency (~0.2-0.3%), and a high percentage of ESC-like colonies among the total colonies (~87.5%), indicating enhanced kinetics and reprogramming efficiency. Therefore, the combined method established in this study provides a valuable platform for the generation and expansion of clinically safe (i.e., integration- and xeno-free) iPS cells, facilitating immune-matched cell therapy in the near future.

2016-04-21 | GSE68035 | GEO

Reliability of 3D Cortical Spheroids Generation from Human Pluripotent Stem Cells

Project description:Here we assess the reliability of human cortical spheroid differentiation cultured in xeno-free, feeder-free conditions. We find high reliability in differentiation across multiple hiPSC lines as well as across experiments, and we anticipate that this directed differentiation approach will be useful for large scale generation of brain-region specific spheroids and disease modeling.

2018-11-06 | GSE120700 | GEO

Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture

Project description:Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propagation of hPS cells on uncoated tissue-culture plastic. The medium consists of the Essential 8 (E8) formulation supplemented with Inter-α-inhibitor (IαI), a human serum-derived protein, recently demonstrated to activate key pluripotency pathways in mouse PS cells. IαI efficiently induces attachment and long-term growth of both embryonic and induced hPS cell lines when added as a soluble protein to the medium at seeding. IαI-supplementation efficiently supports adaptation of feeder-dependent hPS cells to xeno-free conditions, clonal growth as well as single-cell survival in the absence of Rho-associated kinase inhibitor (ROCKi). This time-efficient and simplified culture method paves the way for large-scale, high-throughput hPS cell culture, and will be valuable for both basic research and commercial applications.

2016-06-02 | GSE82103 | GEO

Microarray expression data from mouse embryonic stem cells differentiated into Nkx2-1+ lung and thyroid progenitors

Project description:The in vitro directed differentiation of pluripotent stem cells (PSCs) through stimulation of developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. We used microarrays to detail the global transcriptomes of mouse embryonic stem cells differentiated in vitro into putative thyroid vs lung epithelial lineages using serum-free media supplemented with either BMP4+FGF2 (thyroid media) or BMP4+Wnt3a (lung media.) Differentiated cells carried an Nkx2-1mCherry knock-in reporter to allow sorting of mCherry + vs - cells in each condition on day 14 of differentiation for global transcriptomic profiling.

2017-10-31 | GSE92916 | GEO

Bulk RNAseq anaysis of PSCs and differentiating cells under on-feeder and feeder-free cultures

Project description:Bulk RNAseq anaysis of PSCs and differentiating cells under on-feeder and feeder-free cultures

| PRJNA801842 | ENA

A stress-reduced passaging technique improves the viability of human pluripotent cells

Project description:Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The new method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications. Furthermore, the method did not alter the PSC characteristics after long-term culture and attenuated the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and improves the quality control of human PSC research and applications.

2022-01-23 | GSE184071 | GEO

DMSO-free chemically defined cryopreservation of human mesenchymal stem cells and chondrocytes

Project description:Serum- and xeno-free cryopreservation is suitable for cells to be used in clinical applications. However, dimethyl sulfoxide (DMSO), which is often used in conventional cryoprotectant medium, has been shown to be toxic to many cell types and can induce morphological and gene expression changes that can lead to changes in cell function when they are cultured. In order to evaluate this issue in depth, we examined the efficiency of a DMSO-free/serum-free/xeno-free chemically defined cryoprotectant media, STEM-CELLBANKER DMSO-Free, when culturing human mesenchymal stem cells (MSCs) and chondrocytes. Interestingly, the cells cryopreserved in STEM-CELLBANKER DMSO-Free had a similar growth rate, morphology, and global gene expression profile compared to that observed for cells preserved in conventional freezing medium, which contains 10% DMSO and 20% fetal bovine serum. From these data, we therefore propose that STEM-CELLBANKER DMSO-Free may be an effective tool for maintaining the cellular characteristics of MSCs and chondrocytes during cryo-storage prior to clinical application.

2019-03-22 | GSE79496 | GEO

Genome binding/occupancy profiling of Mll1 in mouse embryo stem cells

Project description:This study describes the epigenetic profiling of Mll1 in mouse ES cells cultures in LIF/serum/feeder-free condition. ChIP-Seq profile of Mll1 was generated in E14Tg2a (E14) mESCs. A sequence profile of genomic DNA (Input) is also included.

2016-02-19 | E-GEOD-66111 | biostudies-arrayexpress

Xeno-free and Chemically Defined Human System for Culturing Human Epidermal Keratinocytes

Project description:Developing an effective xeno-free and defined system for human keratinocyte culture has been one of the fundamental measures in current cellular therapy. For keratinocytes, in particular, such system will not only fulfill clinical safety and quality criteria, it will allow for the management of less severe burns and other skin injuries such as chronic wounds. To date, the expansion of autologous keratinocytes in the clinical settings still relies on 3T3 feeder co-culture system. Here we report a completely xeno-free and defined culture system by using human recombinant laminins as feeder replacement. We have thoroughly characterized the cells cultured in this new system both in vitro and in vivo. We found that laminin-511/-521, and the unanticipated laminin-411/-421, but not -332, -111, -121, -211, or LN-221 support adult human skin keratinocytes and could maintain their self-renewal properties comparable to the 3T3 system. We believe our new culture method should facilitate broader use of cultured epithelial cell products for today’s regenerative medicine.

2018-11-06 | GSE109645 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data