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Single-cell RNA sequencing of vascularized brain organoids

ABSTRACT: Organoids have been widely used as unique models of human brain development and disorders. However, the lack of vasculatures in brain orgnoids limits their application in the study of brain vasculature development and diseases. Here, we described to the generation of vascularized brain organoids (VBOrs) with different brain regions from human embryonic stem cells. The goals of this study are to analyze the cell populations of the new model of vasularized brain organoids cultured from human embryonic stem cells (H9). We found that VBOrs contain variant brain cells inculding neural progenitors, neuronal cells, astrocytes, sparse endothelial cells, and pericytes. The new model of VBOrs should be valuable for addressing questions between brain vasculatures and neural cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE108571 | GEO | 2017/12/28

SECONDARY ACCESSION(S): PRJNA427709

REPOSITORIES: GEO

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Single-cell RNA sequencing of vascularized brain organoids

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