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Comparison of gene expression in the GAS M1T1 strain 5448, wild-type vs pepO mutant

ABSTRACT: Manuscript title: The endopeptidase PepO regulates the SpeB cysteine protease and is essential for the virulence of invasive M1T1 Streptococcus pyogenes. The study investigates the effect of pepO mutation on global gene expression in GAS M1T1 strain 5448.

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE108748 | GEO | 2018/01/05

SECONDARY ACCESSION(S): PRJNA428486

REPOSITORIES: GEO

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Comparison of gene expression in the GAS M1T1 strain 5448, wild-type vs pepO mutant

Project description:Comparison of gene expression in the GAS M1T1 strain 5448, wild-type vs pepO mutant

| PRJNA428486 | ENA

M1T1 Streptococcus pyogenes (isolate 5448) WT and Animal-passaged variant in vitro and in vivo

Project description:Microarray analysis was performed on in vitro and in vivo-derived RNA from the well characterized S. pyogenes strain 5448 WT and animal-passage variant

2010-03-15 | GSE19103 | GEO

M1T1 Streptococcus pyogenes (isolate 5448) WT and Animal-passaged variant in vitro and in vivo

Project description:Microarray analysis was performed on in vitro and in vivo-derived RNA from the well characterized S. pyogenes strain 5448 WT and animal-passage variant Cyclic design with dye swap and biological replicates

2010-05-05 | E-GEOD-19103 | biostudies-arrayexpress

Streptococcus pyogenes strain:5448

Project description:Streptococcus pyogenes 5448 Tn-seq

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Bioplatforms Australia: Antibiotic Resistant Sepsis Pathogens Framework Initiative - Streptococcus pyogenes 5448

Project description:The Antibiotic Resistant Sepsis Pathogens Framework Initiative aims to develop a framework dataset of 5 sepsis pathogens (5 strains each) using an integrated application of genomic, transcriptomic, metabolomic and proteomic technologies. The pathogens included in this initiative are: Escherichia coli, Klebsiella pneumoniae complex, Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus pneumoniae. This submission pertains to strain 5448.

2021-07-01 | GSE152826 | GEO

Streptococcus pyogenes strain:5448

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Group A Streptococcus M1T1 Intracellular Infection of Primary Tonsil Epithelial Cells Dampens Levels of Secreted IL-8 Through the Action of SpyCEP

Project description:Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host immune defenses. However, the signaling pathways and innate immune responses of epithelial cells to GAS are not well understood. In this study, we utilized RNAseq to characterize the inflammatory responses of primary human tonsil epithelial (TEpi) cells to infection with the laboratory-adapted M6 strain JRS4 and the M1T1 clinical isolate 5448. Both strains induced the expression of genes encoding a wide range of inflammatory mediators, including IL-8. Pathway analysis revealed differentially expressed genes between mock and JRS4 or 5448-infected TEpi cells, were enriched in transcription factor networks that regulate IL-8 expression, such as AP-1, ATF-2 and NFAT. While JRS4 infection resulted in high levels of secreted IL-8, 5448 infection did not, suggesting that 5448 may post-transcriptionally dampen IL-8 production. Infection with 5448ΔcepA, an isogenic mutant lacking the IL-8 protease SpyCEP, resulted in IL-8 secretion levels comparable to JRS4 infection. Complementation of 5448ΔcepA and JRS4 with a plasmid encoding 5448-derived SpyCEP significantly reduced IL-8 secretion by TEpi cells. Our results suggest that intracellular infection with the pathogenic GAS M1T1 clone induces a strong pro-inflammatory response in primary tonsil epithelial cells, but modulates this host response by selectively degrading the neutrophil-recruiting chemokine IL-8 to benefit infection.

2018-05-08 | GSE107001 | GEO

5448 vs 5448R- in vivo 24h

Project description:Microarray analysis was performed on in vivo-derived RNA from the well characterized GAS strain 5448 and its derivative 5448R-, containing the mutated ropB allele from 5628. We followed a cyclic design that allowed us to compare every condition to each other at least twice, and guaranteed dye swapping to eliminate effects of non-specific binding. This study consists of 4 replicates of 5448 and 3 replicates of 5448R-.

2010-05-15 | E-GEOD-13656 | biostudies-arrayexpress

General retardation of mRNA degradation caused by speB 5’ UTR partial deletion in Streptococcus pyogenes

Project description:The speB gene of Streptococcus pyogenes has a long 5' UTR with unknown functions. Preliminary studies showed that the partial mutation of this region led to altered expression patterns of the speB gene and ropB, its upstream gene. In this study, we used microarray to examine the effect of speB 5' UTR mutation on the S. pyogenes transcriptome.

2015-09-10 | GSE72838 | GEO

Comparison of gene expression changes in Streptococcus pyogenes in the presence of zinc

Project description:Manuscript title: Zinc disrupts central carbon metabolism and capsule biosynthesis in Streptococcus pyogenes. Macrophages and neutrophils release free zinc to eliminate phagocytosed bacterial pathogens. The study investigates the effect of how zinc toxicity affects Streptococcus pyogenes. Therefore, a microarray analysis was performed in S. pyogenes cells to determine gene expression changes when exposed to high levels of zinc. We discovered that a pathway involved in tagatose-6-phosphate metabolism was upregulated when the cells are under zinc stress.

2015-11-30 | GSE63436 | GEO

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