H3K9ac, H3K27ac, H3K27me3 ChIP sequencing and ATAC sequencing of L4-L6 Dorsal Root Ganglia upon spinal or sciatic axonal injury
ABSTRACT: Purpose: compare the epigenetic response of DRG neurons to an axonal injury in the peripheral vs central nervous system by measuring accessible chromatin and several histone marks Results: we found that after peripheral axonal injury there is a much greater number of epigenetic changes than after central spinal injury, and that these epigenetic changes after peripheral axotomy tended toward increased chromatin accessibility. Overall design: Method: ATACseq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq from sciatic L4-L6 DRG tissue 24 hours after the following treatments: a) spinal cord dorsal column axotomy (DCA), b) laminectomy (Lam), c) sciatic nerve axotomy (SNA), and d) sham sciatic nerve surgery (Sham). Pool of sciatic DRGs from 10 mice were used for each ChIPseq sample. Pool of sciatic DRGs from 1 mouse was used for each ATACseq sample. DRG were collected and flash frozen. Biological duplicates and input controls of each surgical paradigm were generated for each histone mark in the ChIPseq experiments. Biological triplicates of each condition were generated for the ATACseq experiments. Please note that each processed data file was generated from each condition (i.e. replicates pooled together) and is available on the corresponding 'rep1' sample records.