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Gene expression changes in Cenococcum geophilum 1.58-Pinus sylvestris ectomycorrhizal roots, sclerotia and extramatrical mycelium compared to Cenococcum geophilum free-living mycelium

ABSTRACT: Illumina HiSeq technology was used to generate mRNA profiles from Cenococcum geophilum ectomycorrhizal Pine roots, sclerotia and extramatrical mycelium compared to free-living mycelium . Ectomycorrhizal pine roots, sclerotia, extramatrical mycelium and control mycelium were harvested after 90 days and used for RNA extraction. Reads of 150bp were generated and aligned to the C. geophilum reference genome (https://genome.jgi.doe.gov/Cenge3/Cenge3.home.html). Overall design: mRNA profiles from Cenococcum geophilum ectomycorrhizal roots, sclerotia, extramatrical mycelium and free-living mycelium were generated by 150bp Illumina HiSeq2000/2500 sequencing. Three biological replicates were sequenced for mycorrhizal, sclerotia and mycelium samples.

OTHER RELATED OMICS DATASETS IN: PRJNA428975PRJNA428756

INSTRUMENT(S): Illumina HiSeq 2000 (Cenococcum geophilum 1.58)

SUBMITTER: Annegret Kohler  

PROVIDER: GSE108866 | GEO | 2018-03-01

SECONDARY ACCESSION(S): PRJNA428975

REPOSITORIES: GEO

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<i>Cenococcum geophilum</i> is an ectomycorrhizal fungus with global distribution in numerous habitats and associates with a large range of host species including gymnosperm and angiosperm trees. Moreover, <i>C. geophilum</i> is the unique ectomycorrhizal species within the clade Dothideomycetes, the largest class of Ascomycetes containing predominantly saprotrophic and many devastating phytopathogenic fungi. Recent studies highlight that mycorrhizal fungi, as pathogenic ones, use effectors in f  ...[more]

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