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Altered genes expression profile of Rep2 mutants in Fission Yeast upon HU treatment.

ABSTRACT: Expression profiles indicate that C-terminal of rep2 is essential for its transactivation activity. Keywords: rep2 mutants cells treated with 8 mM HU for different time points vs wildtype untreated cells

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE10901 | GEO | 2010/01/08

SECONDARY ACCESSION(S): PRJNA107307

REPOSITORIES: GEO

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Altered genes expression profile of Rep2 mutants in Fission Yeast upon HU treatment.

Project description:Expression profiles indicate that C-terminal of rep2 is essential for its transactivation activity. Keywords: rep2 mutants cells treated with 8 mM HU for different time points vs wildtype untreated cells We analyzed 40 arrays for rep2 mutants cells treated with 8 mM HU to wild type cells.

2010-05-16 | E-GEOD-10901 | biostudies-arrayexpress

HU treatment of Sulfolobus solfataricus P2

Project description:Hydroxyurea (HU) is toxic to Sulfolobus cells. To address the basis of the HU toxicity, we performed transcriptome analyses on untreated cells and cells following exposure to 5 mM HU for 4 hours.

2016-10-05 | GSE87612 | GEO

CEA SGF:E00013#yap1 aft W303a HU response

Project description:Analysis of the response to hydroxyurea in a yeast aft1 mutant strain compared to wild-type strain (BY4741 or BY4742 backgrounds). Cells were grown in YPD rich medium containong 200 mM hydroxyurea (HU) for 2 hours. Analysis of the response to hydroxyurea in a yeast aft1aft2 mutant strain (Y18aft2d) compared to wild-type strain (CM3260). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes due to the aft1 mutation were also analysed in absence of HU (BY4741 background). Analysis of mid-term response to hydroxyurea in a yeast wild-type strain (W303a). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes were analysed compared to the same strain grown in the same medium without HU. Keywords: repeat sample

2005-12-01 | GSE1914 | GEO

RNAs binding to the nucleoid protein HU in Escherichia coli

Project description:HU is a conserved prokaryotic nucleoid-associated protein known for its role in DNA binding and maintaining negative supercoils in DNA. HU also binds to a few RNAs but the natural targets are unknown. To understand the biology of HU interactions with RNA in Escherichia coli, potential HU-RNA in vivo complexes were immunoprecipitated and bound RNAs were examined by hybridization to whole-genome tiling arrays. We observed HU associations with rRNA, multiple tRNAs, a few sRNAs and a small, distinct set of mRNAs. More importantly, we also identified associations between HU and ten intragenic non-coding (nonc) RNAs, two of which are homologous to the annotated Bacterial Interspersed Mosaic Elements (BIME) and boxC elements; the other eight RNAs have not been annotated. We confirmed binding of HU to BIME RNA in vitro. Since HU and some unidentified RNAs have been implicated in the nucleoid structure, we explored an idea that HU, along with some or all of the nonc RNA species discovered here bind to BIME and/or boxC repeats to condense chromosomal DNA in a specific fashion. We propose that nonc RNAs may interact with both HU and DNA repeats to form ternary complexes, which are the anchoring points separating different chromosomal domains.

2012-12-07 | GSE28565 | GEO

Yeast proteomic analysis upon Trp uptake

Project description:untargeted global Mass Spectrometry-based Proteomics in ymr1∆ inp53∆ mutants released from a G1 into HU in AAA-imbalanced (-Trp) or AAA-balanced (+Trp) YPD medium

2021-07-08 | PXD022933 | Pride

HU treated cells vs wildtype untreated cells (asynchronized cells)

Project description:DNA replication checkpiont kinase rad3 (human ATM/ATR-like kinase) and cds1 (human CHK2-like kinase) are known to restore stalled replication forks and prevent subsequent cell cycle progression during a replication block imposed by HU-treatment in fission yeast. In order to identify cell cycle-specific exression which are modulated by replication checkpoint kinase rad3 and cds1, microarray approach was used to profile asynchronous and synchronous cells of wildtype and mutants treated and untreated with HU Keywords: HU treated cells vs wildtype untreated cells

2007-12-20 | GSE4280 | GEO

Ovis aries strain:Hu sheep 1 | breed:Hu sheep

Project description:Hu sheep adipose RNA seq

| PRJNA576088 | ENA

Kc167 ORC Localization in hydroxyurea (HU)

Project description:Determination of the genome-wide distribution of ORC by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to test whether the ORC binding distribution remained the same between G1 and S. ChIP-Chip of ORC in HU compared to input genomic DNA. Biological Replicates: 1

2014-12-05 | E-GEOD-63914 | biostudies-arrayexpress

Isolation and characterization of new fission yeast cytokinesis mutants.

Project description: Not available

| S-EPMC1460233 | biostudies-other

Kc167 Mcm2-7 Localization in HU

Project description:Determination of the genome-wide distribution of the Mcm2-7 helicase by chromatin immunoprecipitation in the Drosophila Kc167 cell line at the beginning of S phase. Kc167 cells were arrested at the G1/S transition with hydroxyurea (HU). Goal was to evaluate changes in the genome-wide Mcm2-7 distribution throughout the cell cycle, and how this relates to the excess Mcm2-7 loaded onto chromatin in G1. ChIP-Chip of Mcm2-7 in HU compared to input genomic DNA. Biological Replicates: 2

2014-11-27 | E-GEOD-63680 | biostudies-arrayexpress

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