ABSTRACT: Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in apple fruits ( Malus x domestica Borkh. ) in four different stages.
Project description:Transcriptional profiling of various apple (Malus x domestica Borkh) organ systems using probes complementary to both sense and anti-sense transcripts. Eight apple organs/samples. Biological replicates: 2 for each sample, independently grown and harvested.
Project description:Transcriptional profiling of various apple (Malus x domestica Borkh) organ systems using probes complementary to both sense and anti-sense transcripts.
Project description:The main objective of this analysis was to sequence the epigenome of the Apple (Malus domestica) doubled haploid 'Golden Delicious' tree. Our secondary objective was to identify differentially methylated regions between DNA purified from leaves and young fruits.
Project description:Closed terminal buds of apple trees (Malus x domestica Borkh, Royal Gala and Castel Gala varieties) grown in commercial orchards were harvested during autumn and winter and exposed to cold treatments
Project description:Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in peach fruits (Prunus persica) in six different stages.
Project description:Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in pear fruits ( "Suli", Pyrus bretschneideri Rdhd. ) in four different stages.
Project description:Closed terminal buds of apple trees (Malus x domestica Borkh, Royal Gala and Castel Gala varieties) grown in commercial orchards were harvested during autumn and winter and exposed to cold treatments 18 biological samples, consisting of 9 pairs of replicates, were analysed in dye-swap. Samples are whole closed terminal buds. Biological replicates are buds from 2 different harvest year subjected to similar cold treatments. Samples with contrasting dormancy status in the same harvest year were compared in 8 dye-swap. Most samples were hybridized more than once in different combinations
Project description:Purpose:The red coloration of apple (Malus × domestica Borkh.) is due to the accumulation of anthocyanins in the fruit peel. Light is essential for anthocyanin biosynthesis in apple.Apple peel can quickly turn red under light conditions after unbagging. Therefore, the implementation of transcriptome sequencing to find genes that promote anthocyanin accumulation in response to light signals is necessary to clarify the mechanism of light-induced anthocyanin accumulation in apple peel.
Project description:The main objective of this analysis was to sequence the epigenome of two apple (Malus domestica) doubled haploid 'Golden Delicious' fruits (GDDH13 and GDDH18). Our secondary objective was to identify differentially methylated regions between DNA purified from the GDDH13 genotype and the GDDH18 genotypes at two developmental stages.
Project description:The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to 4 are allergens present in a variety of different isoforms in apples, with Mal d1 and Mal d3 being the main allergens. In this study, we used proteomics to quantify all four Mal d proteins in 52 apple genotypes with varying allergenic potentials. We revealed the expression of at least 21 Mal d1 isoforms while two Mal d3 and two Mal d4 proteins were found among the genotypes. The allergenic potential of the Mal d isoforms was characterized by comparing isoform abundance with allergenic scores of genotypes (from oral challenge tests). The Mal d peptides detected by comparative analysis presumably have different IgE binding properties and could therefore be used as potential molecular markers to discriminate between hypoallergenic and hyperallergenic cultivars.