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Whole-genome mapping of RUNX1, FUBP1, H3K4me1, H3K4me3, H3K27me3 and H3K27ac in human pre-B lymphoblasts, and B cell precursor leukemia (BCP-ALL)

ABSTRACT: Genome binding/occupancy profiling of human Runt-related transcription factor 1 (RUNX1), Far Upstream Binding Protein 1 (FUBP1) and histone marks by high throughput sequencing. RUNX1 and FUBP1 are two key transcriptional regulators implicated in hematopoiesis, from the maintenance of HSC to lineage-specific differentiation. We hypothesized that both proteins could play a joint role in transcription regulation of hematologic lineages. Here, we investigated the molecular proximity between RUNX1 and FUBP1 proteins on chromatin and their common target genes in pre-B lymphoblasts and B cell precursor leukemia (BCP-ALL). Altogether, our data show that RUNX1 and a subset of FUBP1 colocalize on chromatin in transcriptionally active/accessible chromatin regions mainly in promoters and enhancers regions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE109377 | GEO | 2018/08/21

REPOSITORIES: GEO

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