Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Quantitative Analysis of Srf-regulated Transcriptomes in mouse cardiomyocytes during cardiomyocyte maturation [exp1]

ABSTRACT: The goal of this study is to analyze the role of Srf in transcriptional analysis during cardiomyocyte maturation in mouse

ORGANISM(S): Mus musculus

PROVIDER: GSE109425 | GEO | 2018/09/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Next Generation Sequencing Quantitative Analysis of Srf-regulated Transcriptomes in mouse cardiomyocytes during cardiomyocyte maturation [exp2]

Project description:The goal of this study is to analyze the impact of Srf overexpression on transcription during cardiomyocyte maturation in mouse

2018-09-24 | GSE116030 | GEO

Next Generation Sequencing Quantitative Analysis of Srf-regulated Transcriptomes in mouse cardiomyocytes during cardiomyocyte maturation [exp1]

Project description:Next Generation Sequencing Quantitative Analysis of Srf-regulated Transcriptomes in mouse cardiomyocytes during cardiomyocyte maturation [exp1]

| PRJNA430868 | ENA

Next Generation Sequencing Quantitative Analysis of Srf-regulated Transcriptomes in mouse cardiomyocytes during cardiomyocyte maturation [exp2]

Project description:Next Generation Sequencing Quantitative Analysis of Srf-regulated Transcriptomes in mouse cardiomyocytes during cardiomyocyte maturation [exp2]

| PRJNA476863 | ENA

SRF-null neonatal cardiomyocytes

Project description:Serum Response Factor (SRF) is a transcriptional regulator required for mesodermal development. Numerous studies have implicated SRF as a central regulator of muscle gene expression and myogenesis. In this present study we used a loss of function approach to delineate the role of SRF in cardiac myocyte gene expression and function. In SRF null neonatal cardiomyocytes, we observe severe defects in the contractile apparatus, including Z-disc and stress fiber formation, as well as mislocalization and/or attenuation of sarcomeric proteins. Consistent with this, gene array and RT-PCR analyses show downregulation of genes encoding key cardiac transcriptional regulatory factors and proteins required for the maintenance of sarcomeric structure, function, and regulation. Chromatin IP analysis reveals that at least a subset of these proteins are likely regulated directly by SRF. Together the results presented here reveal new cellular and genetic mechanisms through which SRF exacts control over the contractile apparatus in cardiac myocytes. Keywords: genetic modification

2005-12-21 | GSE3181 | GEO

Sarcomeres regulate murine cardiomyocyte maturation through MRTF-SRF signaling.

Project description: Not available

| S-EPMC7812832 | biostudies-literature

Quantitative Analysis of Cardiomyocyte-specific Tbx20 Overexpression and Control Mouse Heart Transcriptomes Using Next Generation Sequencing

Project description:Purpose: The goals of this study is to determine the differential expression of transcriptome in cardiomyocyte-specific Tbx20 overexpressing adult heart (DTG) compared to controls (STG).

2018-09-02 | GSE72699 | GEO

Small RNA sequencing in mouse cardiomyocytes after siRNA mediated knockdown of Srf

Project description:We performed two independent siRNA mediated knockdowns of Srf (Srf si1 & Srf si2) and an unspecific siRNA (siNon) in mouse cardiomyocytes HL-1 cells. Small RNAs were sequenced by Illumina/Solexa next-generation (single-end) sequencing technology. The sequence reads were mapped to the mouse reference genome (NCBI v37, mm9) using MicroRazerS. MicroRazerS searches for the longest possible prefix-match of each read, i.e. the longest possible contiguous match starting at the first base. Hence, it is robust to possible adapter sequence at the 3' end of a read and requires no adapter trimming. Small RNA-seq profiles of two siRNA mediated knockdowns of Srf and an unspecific siRNA in mouse cardiomyocytes

2011-01-03 | E-GEOD-19066 | biostudies-arrayexpress

MKL1 augments megakaryocyte maturation by enhancing the SRF regulatory axis [RNA-seq]

Project description:SRF is a ubiquitous transcription factor that binds DNA in association with myocardin-family proteins (e.g. MKL1), or the ternary complex factor (TCF) family of ETS proteins. In primary hematopoietic cells, knockout of either SRF or MKL1 decreases megakaryocyte maturation caused thrombocytopenia, and MKL1 over-expression (MKL1OE) in the Human Erythroleukemia (HEL) cell line enhances megakaryopoiesis, but the mechanisms underlying these effects are unknown. To elucidate the role of SRF and MKL1 in megakaryopoiesis, integrated analysis was performed of anti-SRF ChIP-seq and RNA-seq data in undifferentiated and differentiated HEL cells, both with and without induction of MKL1OE. MKL1OE enhances TPA-induced megakaryopoiesis with 25% of genes upregulated, as opposed to 11% with TPA alone. MKL1OE increases SRF binding to genomic sites, and enhances expression of specific target cytoskeletal genes in response to TPA. Genes upregulated in response to TPA are predicted to be regulated by SRF and ETS factors, whereas those upregulated in TPA plus MKL1OE lack ETS binding motifs. Using ChIP-PCR, we validated that both SRF and MKL1 have increased binding at target upregulated genes, e.g. CORO1A, with MKL1OE. We show for the first time that MKL1 increases both the genomic association and activity of SRF, and upregulates genes that enhance megakaryopoiesis.

2018-10-29 | GSE112277 | GEO

MKL1 augments megakaryocyte maturation by enhancing the SRF regulatory axis [ChIP-seq]

2018-10-29 | GSE112265 | GEO

Small RNA sequencing in mouse cardiomyocytes after siRNA mediated knockdown of Srf

2011-01-03 | GSE19066 | GEO