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Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE109478 | GEO | 2018/02/22

REPOSITORIES: GEO

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Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots.

Project description:Remodeling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and reduced representation bisulfite sequencing (RRBS), we, respectively, investigated sRNA expression and DNA methylation in pure fractions of motile spermatozoa collected from young healthy individuals before, after 6 weeks of endurance training and after 3 months without exercise. Expression of 8 PIWI interacting RNA were changed by exercise training. RRBS analysis revealed 330 differentially methylated regions (DMRs) after training and 303 DMRs after the detraining period, which were, in both conditions, enriched at close vicinity of transcription start sites. Ontology analysis of genes located at proximity of DMRs returned terms related to neurological function at the trained state and, to a much lesser extent, at the detrained state. Our study reveal that short-term endurance training induces marked remodeling of the sperm epigenome, and identify genes related to the development of the central nervous system as potential hot spots for epigenetic variation upon environmental stress.

| S-EPMC5785820 | biostudies-literature

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots [RRBS]

Project description:Remodelling of the sperm epigenome by lifestyle factors before conception could account for altered metabolism in the next generation offspring. Here, we hypothesized that endurance training changes the epigenome of human spermatozoa. Using small RNA (sRNA) sequencing and Reduced Representation Bisulfite Sequencing (RRBS), we respectively investigated sRNA expression and DNA methylation in pure fractions of motile spermatozoa collected from young healthy individuals before, after 6 weeks of endurance training and after 3 months without exercise. Expression of 8 piwi interacting RNA were changed by exercise training. RRBS analysis revealed 330 differentially methylated regions (DMRs) after training and 303 DMRs after the detraining period which were, in both conditions, enriched at close vicinity of transcription start sites. Ontology analysis of genes located at proximity of DMRs returned terms related to neurological function at the trained state and, to a much lesser extent, at the detrained state. Our study reveal that short-term endurance training induces marked remodelling of the sperm epigenome, and identify genes related to the development of the central nervous system as potential hot spots for epigenetic variation upon environmental stress.

2018-02-22 | GSE109474 | GEO

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots [sncRNA]

2018-02-22 | GSE109475 | GEO

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

Project description:Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots

| PRJNA431167 | ENA

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots [RRBS]

Project description:Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots [RRBS]

| PRJNA431164 | ENA

Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots [sncRNA]

Project description:Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots [sncRNA]

| PRJNA431173 | ENA

DNA methylation analysis of human skeletal muscle after endurance training

Project description:Regular endurance exercise training induces beneficial functional and health effects in human skeletal muscle. The putative contribution to the training response of the epigenome as a mediator between genes and environment has not been clarified. Here we investigated the contribution of DNA methylation and associated transcriptomic changes in a well-controlled human intervention study. Training effects were mirrored by significant alterations in DNA methylation and gene expression in regions with a homogeneous muscle energetics and remodeling ontology. Differential DNA methylation predominantly occurred in regulatory enhancer regions, where known binding motifs of MRF, MEF2 and ETS proteins were identified. A transcriptional network analysis revealed modules harboring distinct ontologies, and interestingly the overall direction of the changes of methylation within each module was inversely correlated to expression changes. In conclusion, we show that highly consistent and associated modifications in methylation and expression, concordant with observed health-enhancing phenotypic adaptations, are induced by a physiological stimulus.

2014-11-04 | GSE60655 | GEO

Bovine Sperm-borne miRNAs

Project description:Purpose: to construct a library of miRNAs in bovine sperm was constructed using Illumina high‐throughput sequencing technology. Result: Unique sequences that were 18–26 nucleotides in length were mapped to specific precursors in miRBase 20.0 using BLAST. A total of 951 known miRNAs and 8 novel, highly expressed miRNA candidates were identified.

2021-02-26 | GSE167556 | GEO

Skeletal muscle methylation signatures of obese high- and low- responders to endurance exercise training

Project description:The participants performed 8 weeks of superised aerobic endurance exercise. Skeletal muscle biopsise were taken at rest before and after intervention and matched analysis was performed.

2024-04-24 | GSE244359 | GEO