Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of differentiated normal mouse urothelial organoids reveals novel pathways activated during urothelial differentiation

ABSTRACT: Transcriptomic analysis revealed significant changes between organoids growing in full medium growth conditions (proliferative NMU-o) and deprived medium (differentiated NMU-o). Around 4100 genes are differentially expressed between both conditions. Moreover, GSEA analysis in proliferation showed pathways related to cell cycle, DNA repair, RNA biology and protein synthesis; in differentiation, pathways related to cellular traffic, cell-cell adhesion and notch signalling are up-regulated.

ORGANISM(S): Mus musculus

PROVIDER: GSE109566 | GEO | 2019/08/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Neuromedin U promotes a malignant phenotype in luminal breast cancer involving Naked 2 (NKD2) modulated WNT signalling

Project description:Neuromedin U (NMU), which is thought to contribute to putative metastasis processes in various tumor entities, was identified as being up-regulated in breast cancer. Therefore, we aimed to uncover the role of NMU in breast cancer subtypes deciphering for the first time NMU-driven signalling pathways and downstream targets. Stable gain-of-function in vitro models were generated to decipher the biological role of NMU in breast cancer cells. To uncover underlying signalling pathways and key molecules affected by NMU in the luminal-like SKBR3 model, microarray analysis was carried out.

2017-08-13 | GSE75932 | GEO

Transcriptome analysis of differentiated normal mouse urothelial organoids reveals novel pathways activated during urothelial differentiation

Project description:Transcriptome analysis of differentiated normal mouse urothelial organoids reveals novel pathways activated during urothelial differentiation

| PRJNA431400 | ENA

Influence of VEGF stimulation and serum deprivation on gene activation patterns of human adipose tissue-derived stromal cells

Project description:ASCs cultured in complete medium, ASCs cultured in serum serum-deprived medium, and ASCs stimulated with VEGF in serum-deprived medium were compared. Using microarray analysis, gene expression from the whole genome was compared between conditions. Compared to ASCs in complete medium, expression of 190 and 108 ASC genes were significantly regulated altered by serum deprivation and serum deprivation combined with VEGF, respectively. No significant differences in gene expression patterns between serum-deprived ASCs and serum-deprived ASC combined with VEGF stimulation were found. Genes most prominently and significantly up-regulated by both conditions were growth factors (IGF1, BMP6, PDGFD, FGF9), adhesion molecule CLSTN2, extracellular matrix related proteins like matricellular proteins SMOC2, SPON1 and ADAMTS12, and inhibitors of proliferation (JAG1). The most significantly down-regulated genes included matrix metalloproteinases (MMP3, MMP1), and proliferation markers (CDKN3) and GREM2, - a BMP6 antagonist.

2015-03-20 | E-MTAB-3066 | biostudies-arrayexpress

Single cell RNA-Seq of Proliferative (NMU-O-P) and Differentiated (NMU-O-D) Normal Mouse Urothelium Organoids

Project description:Single cell RNA-Seq of Proliferative (NMU-O-P) and Differentiated (NMU-O-D) Normal Mouse Urothelium Organoids

| PRJNA545302 | ENA

Rat mammary normal - tumor

Project description:Rat mammary tumors induced by N-methyl-N-nitrosurea (NMU) and normal mammary gland Keywords = breast cancer Keywords = rat mammary tumor Keywords = NMU Keywords = animal model. Keywords: other

2005-04-30 | GSE1872 | GEO

Differentiated human airway organoids to assess infectivity of emerging influenza virus.

Project description: Not available

| S-EPMC6042130 | biostudies-literature

Transcription profiling of rat strains Cop AND F334 mammary gland treated WITH N-nitrosomethylurea (NMU), TIME series

Project description:Gene expression profiles in mammary glands of different rat strains are genetically defined. Experiment Overall Design: 18 Cop rats with or without N-nitrosomethylurea (NMU) treatment at 6h, 1d, and 30 d compared to 18 F344 rats with or without NMU treatment at 6h, 1d, and 30 day

2008-04-07 | E-GEOD-10641 | biostudies-arrayexpress

Mouse and human urothelial cancer organoids: A tool for bladder cancer research.

Project description: Not available

| S-EPMC6410883 | biostudies-literature

Gene expression of UC organoids and UC model organoids (long-term inflammation model) derived from human colonic organoids

Project description:Gene expression changes of human colon orgnaoids by 60weeks of inflammatory stimulation in vitro (long-term inflammed organoids) and after 10weeks from the removal of stimulation (inflammation-removed organoids). GSEA analysis was performed about RNA1-9 (Organoids #1). For the reproducibility of the analysis, GSEA was performed in triplicate. The key molecule for long-term inflammation was extracted from RNA10-12 (Organoids #2). The comparison of gene expression of organoids derived from normal(RNA1-3) and UC mucosa(RNA13-15). For the reproducibility, the analysis was performed in triplicate.

2020-10-26 | GSE156806 | GEO

Transcription profiling of rat mammary tumors induced by N-methyl-N-nitrosurea (NMU) and normal mammary gland

Project description:Rat mammary tumors induced by N-methyl-N-nitrosurea (NMU) and normal mammary gland

2007-09-22 | E-GEOD-1872 | biostudies-arrayexpress