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Sensitivity of MiaPaCa2 cells to E2F inhibitor Ly101-4B.

ABSTRACT: MiaPaCa2 cells where screened with a set of original compounds with anticancer activity available at our laboratory. From these compounds we identified the nucleoside analogue ly101-4B as a strong inhibitor of the E2F activity. We confirmed its activity by studying its effect on MiaPaCa2's cell viability , caspase 3/7 activity and LDH release, to measure cell growth, apoptosis and necrosis after treatment with LY101-4B 25 µM. Then, to investigate the mechanism of action of ly101-4B we performed a transcriptomic analysis by using an Affymetrix approach (Genechip® Human Gene 1.0 ST Arrays).

ORGANISM(S): Homo sapiens

PROVIDER: GSE109853 | GEO | 2019/02/28

REPOSITORIES: GEO

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