Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from primary vascular smooth muscle cells (VSMCs) from 5 unrelated patients - comparing different treatments versus untreated controls

ABSTRACT: This study explores the effect of WNT5A signaling on the vascular redox state in obesity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE109859 | GEO | 2021/02/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression data from primary vascular smooth muscle cells (VSMCs) from 5 unrelated patients - comparing different treatments versus untreated controls

Project description:Expression data from primary vascular smooth muscle cells (VSMCs) from 5 unrelated patients - comparing different treatments versus untreated controls

| PRJNA432204 | ENA

mRNA expression profile in vascular smooth muscle cells (VSMCs) after inducing calcification

Project description:Vascular calcification (VC) is often associated with cardiovascular and metabolic diseases. However, the molecular mechanisms linking VC to these diseases have yet to be elucidated. Here we report that MDM2-induced polyubiquitination of histone deacetylase 1 (HDAC1) mediates VC. Loss of HDAC1 activity via either chemical inhibitor or genetic ablation enhanced VC. HDAC1 protein, but not mRNA, was reduced in cell and animal calcification models and in human calcified coronary artery. In the calcification-provoking condition, proteasomal degradation of HDAC1 preceded VC. The calcification-provoking condition induced MDM2 E3 ligase, which then resulted in HDAC1 K74 polyubiquitination. Overexpression of MDM2 enhanced VC, whereas loss of MDM2 blunted it. Decoy peptides spanning HDAC1 K74 and RG 7112, an MDM2 inhibitor, prevented VC in vivo and in vitro. These results demonstrate a previously unknown ubiquitination pathway and suggest MDM2-mediated HDAC1 polyubiquitination as a new therapeutic target in VC. Calcification was induced in rat aorta vascular smooth muscle cells with inorganic phosphate (Pi). Total RNA were extracted from the cells 3 and 6 days later. mRNA profile of the sample was compared with normal control.

2015-11-07 | E-GEOD-74755 | biostudies-arrayexpress

microRNA expression profile in vascular smooth muscle cells (VSMCs) after inducing calcification

Project description:Vascular calcification, the ectopic deposition of calcium in blood vessels, develops in association with various metabolic diseases and atherosclerosis. Because it often causes stiffness and remodeling of the blood vessels, vascular calcification increases morbidity and mortality. Both miRNA and mRNA microarrays (Series GSE74755) were performed with rat VSMCs and reciprocally regulated pairs of miRNA and mRNA were selected after bioinformatic analysis.

2019-12-31 | GSE130486 | GEO

mRNA expression profile in vascular smooth muscle cells (VSMCs) after inducing calcification

2015-11-07 | GSE74755 | GEO

MT1-MMP (MMP14)-dependent gene expression in APOE-null vascular smooth muscle cells (VSMCs)

Project description:VSMC-specific MT1-MMP gene targeting in APOE-null mice promotes atherosclerosis and iliac artery aneurysm formation. To determine the MT1-MMP-dependent regulation of VSMC function, whole-genome transcriptomes of wild-type and MT1-MMP-null APOE-null VSMCs were determined. We used microarray-based transcriptome analysis to detect MT1-MMP-dependent regulation of VSMC function under atherogenic conditions.

2017-08-01 | GSE100661 | GEO

Effect of depletion of h3f3b on gene expression in vascular smooth muscle cells (VSMCs).

Project description:To investigate the function H3f3b in the regulation of VSMC phenotypic transition, we infect VSMCs with shNT or shH3f3b virus.

2024-04-06 | GSE229125 | GEO

Gene expression profiling of miR-128 overexpression in Primary Mouse Vascular Smooth Muscle Cells (VSMCs)

Project description:MicroRNAs (miRNAs) are small non-coding RNAs that modulate gene expression by negatively regulating translation of target genes. miRNAs involvement in vascular smooth muscle cell (VSMC) biology has been deeply investigated, nevertheless the role of miR-128 in such context has not been yet explored. We aimed to evaluate whether this miR-128 might modulate VSMC phenotype similarly to what was previously observed for other type of contractile cells, such as cardiomyocytes (CM) and skeletal muscle cells. To this end, we compared the expression profiles of miR-128 overexpression in Primary mouse VSMCs and control groups (treated with scrambled miRNA). The expression profiles were defined by Illumina arrays.

2020-09-07 | GSE124764 | GEO

RNA expression profiles of mouse vascular smooth muscle cells (VSMCs) transfected with scramble siRNA (scr) and circEsyt2 siRNA (si-circ)

Project description:Circular RNAs play an important role in multiple pathological conditions. Recently, we identified a novel circRNA-circEsyt2 from mouse atherosclerosis (AS) model and found its involvement in AS development. To explore the molecular mechanism of circEsyt2, we performed RNA-seq to investigate the regulation of circEsyt2 on mRNA profiles.

2021-12-31 | GSE133270 | GEO

microRNA expression profile in vascular smooth muscle cells (VSMCs) after inducing calcification

Project description:microRNA expression profile in vascular smooth muscle cells (VSMCs) after inducing calcification

| PRJNA540465 | ENA

Phosphodiesterase 3A (PDE3A) deletion suppresses proliferation of cultured murine vascular smooth muscle cells (VSMCs) via inhibition of mitogen-activated protein kinase (MAPK) signaling and alterations in critical cell cycle regulatory proteins.

Project description: Not available

| S-EPMC3138244 | biostudies-literature