TSS identification of Rap1-regulated transcripts by 5' end RNA sequencing

ABSTRACT: Eukaryotic cells utilize several mechanisms to ensure that expression of aberrant non-coding RNAs is limited. Gene looping, chromatin modification or remodeling, and RNA surveillance contribute to ensure the fidelity of transcription and limit non-coding transcripts. We have identified that the transcription factor Rap1 is critical for limiting the expression of aberrant RNAs, particularly near the highly expressed ribosomal protein genes. Here we utilize a 5’ end RNA sequencing protocol to identify transcript TSSs at single nucleotide resolution in wild-type and Rap1 auxin-inducible degron strains of Saccharomyces cerevisiae.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE110000 | GEO | 2018/12/20

REPOSITORIES: GEO