Profiling of alternative transcription start sites and alternative polyadenylation sites in Xenopus tropicalis

ABSTRACT: We have developed both WTSS-seq (whole transcriptome start site sequencing) and WTTS-seq (whole transcriptome termini site sequencing) methods to capture either 5’- or 3’-ends of transcripts. HATT-seq (head and tail tag sequencing) is still under development, which can be used to capture both 5’- and 3’ ends of each transcript simultaneously. Iso-seq was used to produce full-length transcripts, which can be used to validate both alternative transcription start sites and alternative polyadenylation sites. CAGE-seq was used to confirm alternative transcription start sites only.

ORGANISM(S): Xenopus tropicalis

PROVIDER: GSE110063 | GEO | 2020/02/01

REPOSITORIES: GEO