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Base editing with a Cpf1–cytidine deaminase fusion

ABSTRACT: The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich PAM sequences. To overcome this limitation, we developed a CRISPR/Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and converts C to T in human cells with low levels of indels, non-C-to-T substitutions and off-target editing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE110136 | GEO | 2018/03/18

REPOSITORIES: GEO

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