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Comparison between WT and bes1 in an in vitro tissue culture system, VISUAL

ABSTRACT: We have previously established an in vitro tissue culture system (named VISUAL; Kondo et al., 2016), in which xylem and phloem differentiation can be induced with Arabidopsis thaliana cotyledons To compare gene expression profiles between WT and bes1 during vascular development, we performed GeneChip analysis using VISUAL.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE110199 | GEO | 2018/02/07

REPOSITORIES: GEO

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Cell-sorting analysis with SEOR1pro::SEOR1-YFP in a novel in vitro tissue culture system, VISUAL

Project description:We established a novel in vitro tissue culture system (named VISUAL), in which xylem and phloem differentiation can be induced with Arabidopsis thaliana cotyledons To isolate phloem-specific genes in VISUAL, we performed GeneChip analysis after cell-sorting experiments with SEOR1pro::SEOR1-YFP.

2016-05-25 | GSE80027 | GEO

The effects of different types of sugars on vascular cell diffentiation in a tissue culture system, VISUAL

Project description:We established a novel in vitro tissue culture system (named VISUAL), in which xylem and phloem differentiation can be induced with Arabidopsis thaliana cotyledons To investigate the effects of differenet sugars in VISUAL, we performed GeneChip analysis .

2023-05-16 | GSE223284 | GEO

Comparison between WT and apl in a novel in vitro tissue culture system, VISUAL

Project description:We established a novel in vitro tissue culture system (named VISUAL), in which xylem and phloem differentiation can be induced with Arabidopsis thaliana cotyledons To compare gene expression profiles between WT and apl during vascular development, we performed GeneChip analysis using VISUAL.

2016-05-25 | GSE80026 | GEO

Six-hour interval time-course expression data during VISUAL

Project description:We used in vitro tissue culture system (named VISUAL), in which xylem and phloem differentiation can be induced with Arabidopsis thaliana cotyledons To investigate temporal gene expression profiles during ectopic vascular cell defferentiaction processes, we performed GeneChip analysis using VISUAL.

2021-05-19 | GSE171548 | GEO

Comparison among samples subjected to a novel in vitro tissue culture system, VISUAL-CC

Project description:We established a novel in vitro tissue culture system (named VISUAL-CC), in which phloem companion cell (CC) differentiation can be induced with Arabidopsis thaliana cotyledons. To compare gene expression profiles between VISUAL and VISUAL-CC, we conducted GeneChip analysis using two different in vitro cultures. CC-S means a sample that strongly induces CC differentiation. CC-M means a sample that moderately induces CC differentiation. V means a VISUAL sample, that does not induce CC differentiation at all.

2020-03-31 | GSE141037 | GEO

Comparison between WT and bes1 in an in vitro tissue culture system, VISUAL

Project description:Comparison between WT and bes1 in an in vitro tissue culture system, VISUAL

| PRJNA433158 | ENA

Analysis of BES1-PIF4 interdependency in gene expression

Project description:BES1 transcription factor is a master regulator of brassinosteroid signalling. Part of BES1 activity as a transcription factor is brought about upon dimerization with light-responsive PIF4 transcription factor. To analyse BES1-dependent transcriptional cascade, and to which extent BES1 requires PIF4/PIF5 for target gene regulation, we performed microarray analysis of the following phenotypes: Col-0, bes1-D, pif4pif5 and bes1-D;pif4pif5 in dark grown seedlings. Col-0, bes1-D, pif4pif5 and bes1-D;pif4pif5 5-days seedlings grown in the dark were used for RNA extraction. 3 independent biological samples were collected for each genoptye.

2016-08-05 | E-GEOD-85211 | biostudies-arrayexpress

Analysis of BES1-PIF4 interdependency in gene expression

2016-08-05 | GSE85211 | GEO

Genome-wide mapping of BES1 binding sites in Arabidopsis thaliana

Project description:Brassinosteroids (BRs) are important regulators for plant growth and development. BRs signal to control the activities of the BES1 and BZR1 family transcription factors. In order to further understand the mechanism by which BES1/BZR1 regulates downstream genes, we performed chromatin immunoprecipitation coupled with tiling arrays (ChIP-chip) to identify BES1 binding sites in the genome. By combining ChIP-chip data with gene expression microarray data, we are able to discover genes that are directly regulated by BES1 (i.e. BES1 target genes). Chromatin from bes1-D Arabidopsis seedlings are immunoprecipitated by anti-BES1 antibody with anti-GFP antibody as control.

2011-01-02 | E-GEOD-24684 | biostudies-arrayexpress

Genome-wide mapping of BES1 binding sites in Arabidopsis thaliana

2011-01-02 | GSE24684 | GEO

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