Transcriptomics,Genomics

Dataset Information

38

Expression profiling in exosomal miRNAs of human bone marrow stromal cells derived from multiple myeloma patients


ABSTRACT: Bone marrow stromal cells (BMSCs) and their exosomes are a promising area of cancer therapy. Multiple myeloma (MM) is refractory hematologic malignancy. Bone marrow stromal cells (BMSCs) interact with MM cells in the bone marrow (BM), and also create a permissive microenvironment for MM cell growth and survival. Recent evidence indicated that exosome-mediated MM cell-BMSC communication plays an important role in the MM microenvironment. In this study, we investigated the biological property of the exosomes and exosomal miRNAs derived from BMSCs, aiming to establish the emerging strategies to target MM microenvironment to prevent tumor growth and spread. Overall design: Human BMSCs of elderly (68 and 72 years old) healthy donors were purchased from Lonza (Normal-BMSCs). BM samples were obtained from multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) patients in accordance with the Declaration of Helsinki and using protocols approved by the research Ethics Committee of Tokyo Medical University (IRB No. 2648), and BMSCs derived from MM patients (MM-BMSCs) and MGUS patients (MGUS-BMSCs) were isolated using the classical plastic adhesion method. The exosomes from culture medium of BM-MSCs were isolated by Exoquick-TC (SBI). Total RNA from each exosome fractions (2 Normal-BMSCs, 6 MM-BMSCs and 3 MGUS-BMSCs) was extracted by the miRneasy mini kit (Qiagen). The exosomal miRNA profiling was done using a TaqMan low-density array (ABI), and Student’s t-test was used to determine statistical significance for comparisons between young and old groups using R software.

INSTRUMENT(S): Taqman Array Human MicroRNA A Card v2.0

SUBMITTER: Tomohiro Umezu  

PROVIDER: GSE110271 | GEO | 2018-06-08

REPOSITORIES: GEO

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