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Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-Seq II]

ABSTRACT: We report single cell RNA-seq data that reveals that Embryonic Multipotent Progenitors (EMPs) in the mammary gland express a unique hybrid basal and luminal signature associated with the different adult lineages.

ORGANISM(S): Mus musculus

PROVIDER: GSE110351 | GEO | 2018/06/04

REPOSITORIES: GEO

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Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]

Project description:The mammary gland (MG) is composed of basal cells (BCs) and luminal cells (LCs). While it is generally believed that MG arises from embryonic multipotent progenitors (EMPs), it remains unclear when lineage restriction occurs and what are the mechanisms responsible for the switch from multipotency to unipotency during MG morphogenesis. Here, we performed multicolor lineage tracing and assessed the fate of single progenitors and demonstrated the existence of a developmental switch from multipotency to unipotency during embryonic MG development. Molecular profiling and single cell RNA-seq revealed that EMPs express a unique hybrid basal and luminal signature and the factors associated with the different lineages. Sustained p63 expression in EMPs promotes unipotent BC fate and was sufficient to reprogram adult LCs into BCs by promoting an intermediate hybrid multipotent like state. Altogether, this study identifies the timing and the mechanisms mediating the early lineage segregation of multipotent progenitors during MG development.

2018-06-04 | GSE109707 | GEO

Early lineage segregation of multipotent embryonic mammary gland progenitors [expression]

Project description:The mammary gland (MG) is composed of basal cells (BCs) and luminal cells (LCs). While it is generally believed that MG arises from embryonic multipotent progenitors (EMPs), it remains unclear when lineage restriction occurs and what are the mechanisms responsible for the switch from multipotency to unipotency during MG morphogenesis. Here, we performed multicolor lineage tracing and assessed the fate of single embryonic progenitors during mouse MG development. We demonstrated the existence of a developmental switch from multipotency to unipotency during embryonic MG development. Molecular profiling and single cell RNA-seq revealed the hybrid gene expression of EMPs, and the gene trajectory and lineage segregation occurring during MG development. In situ characterization showed that one of the earliest signs of lineage segregation consists in the restricted expression of p63 in the future BCs. Sustained p63 expression during MG development promotes unipotent BC fate in EMPs. Altogether, this study identifies the timing and the mechanisms mediating the switch from multipotency to unipotency during MG development. To understand the molecular mechanisms regulating multipotency during embryonic development, we isolated EMPs by FACS at E14, performed their transcriptional profiling by microarray and compared their transcriptome to adult BCs and LCs

2018-06-04 | GSE109306 | GEO

Early lineage segregation of multipotent embryonic mammary gland progenitors

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors

| PRJNA431774 | ENA

Early lineage segregation of multipotent embryonic mammary gland progenitors.

Project description: Not available

| S-EPMC5985933 | biostudies-literature

Early lineage segregation of multipotent embryonic mammary gland progenitors [expression]

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors [expression]

| PRJNA430395 | ENA

Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-Seq II]

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-Seq II]

| PRJNA433520 | ENA

Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]

| PRJNA431773 | ENA

Early lineage segregation of multipotent embryonic mammary gland progenitors

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-06-04 | GSE109711 | GEO

AP-2γ is Required for Maintenance of Multipotent Mammary Stem Cells

Project description:Mammary gland ductal morphogenesis depends on the differentiation of mammary stem cells (MaSCs) into basal and luminal lineages. The AP-2γ transcription factor, encoded by Tfap2c, has a central role in mammary gland development but its effect in mammary lineages and specifically MaSCs is largely unknown. Herein, we utilized an inducible, conditional knockout of Tfap2c to elucidate the role of AP-2γ in maintenance and differentiation of MaSCs. Loss of AP-2γ in the basal epithelium profoundly altered the transcriptomes and decreased the number of cells within several clusters of mammary epithelial cells, including adult MaSCs and luminal progenitors. AP-2γ regulated the expression of genes known to be required for mammary development including Cebpb, Nfkbia, and Rspo1. As a result, AP-2γ-deficient mice exhibited repressed mammary gland ductal outgrowth and inhibition of regenerative capacity. The findings demonstrate that AP-2γ can regulate development of mammary gland structures potentially regulating maintenance and differentiation of multipotent MaSCs.

2020-12-30 | GSE143159 | GEO

Single Cell RNA-sequencing of mammary gland epithelial cells

Project description:The mammary gland epithelium is composed of basal cells (BC) and luminal cells (LC). Lineage tracing demonstrates that many glandular epithelia initially develop from multipotent basal stem cells (BaSCs) that are replaced in adult life by distinct pool of unipotent stem cells. However, adult unipotent BaSC can reactivate multipotency and give rise to LCs upon transplantation or oncogene expression, demonstrating the important plasticity of BaSCs in regenerative and pathological conditions, and suggesting that an active mechanism restricts multipotency in BaSCs during physiological conditions. Here, we assess whether basal and luminal cell-cell communication restricts multipotency in glandular epithelia.

2020-04-23 | GSE148791 | GEO

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