Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translatome of developing drosophila t4/t5 neurons in wildtype versus atonal loss of function genotypes

ABSTRACT: Atonal is a proneural transcription factor expressed in the Drosophila neuroblast cluster known as the inner proliferation center (IPC). The t4/t5 neurons that derived from the IPC require atonal for their normal development. To characterize the role of Atonal in t4/t5 development we have sequenced the translated RNA in t4/t5 neurons in wild type and ato loss of function flies.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE110705 | GEO | 2018/02/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Chromatine immunoprecipation of atonal binding sites in Drosophila L3 Brains

Project description:Atonal is a proneural transcription factor expressed in the Drosophila neuroblast cluster known as the inner proliferation center (IPC). To characterize the putative targes of Atonal in the IPC we have used an endogenously GFP-tagged version of atonal to immunoprecipate brain samples with anti-GFP antibodies

2018-02-21 | GSE110687 | GEO

mRNA-seq of nuclear RNA extracted from T4 and T5 neurons of D. melanogaster

Project description:T4 and T5 neurons are components of the neuronal circuit for motion vision in flies. To identify genes involved in neuronal computation of T4 and T5 neurons, we perfomed transcriptome analysis. Nuclei of T4 and T5 neurons were immunoprecipitated, total RNA was harvested and used for mRNA-seq with Illumina technology. In two biological replicates, we mapped 154 and 119 million reads to D. melanogaster genome. mRNA-seq provided information about expression levels of 17,468 annotated transcripts in the T4 and T5 neurons.

2016-09-30 | GSE77198 | GEO

mRNA-seq of nuclear RNA extracted from T4 and T5 neurons of D. melanogaster

2016-09-30 | E-GEOD-77198 | biostudies-arrayexpress

Translatome of developing drosophila t4/t5 neurons in wildtype versus atonal loss of function genotypes

Project description:Translatome of developing drosophila t4/t5 neurons in wildtype versus atonal loss of function genotypes

| PRJNA434323 | ENA

The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons

Project description:A challenge in systems biology is to understand the gene regulatory networks that connect early cellular specification to terminal differentiation of specific cell types. In neurogenesis, neural specification has been well studied, but the link between the proneural transcriptional regulators of specification and the genes that must be activated to construct differentiated neurons is obscure. High resolution temporal profiling of gene expression reveals the events downstream of Atonal (Ato) proneural gene function in Drosophila sensory neurons. Unexpectedly, many differentiation genes are activated soon after specification, even before cell cycle exit and overt neuronal differentiation. Prominent among them are genes required for construction of the ciliary dendrite. Ato activates differentiation both directly and indirectly via several intermediate transcriptional regulators, including Rfx and a new Forkhead family factor. Our analysis of these factors and their regulation provides insight into how proneural factors regulate neuronal subtype differentiation. Investigating the molecular mechanisms of peripheral nervous sytem development in Drosophila melanogaster. Affymetrix Drosophila version 2.0 chips used to measure gene expression from GFP+ and GFP- cells from embryos expressing GFP under the control of the atonal gene enhancer in both wild type and mutant embryos. Data generated for three developmental time-points in quadruplicate.

2011-02-18 | E-GEOD-21520 | biostudies-arrayexpress

A combinatorial code of transcription factors specifies subtypes of visual motion-sensing neurons in Drosophila

Project description:Direction-selective T4/T5 neurons exist in four subtypes, each tuned to visual motion along one of the four cardinal directions. Along with their directional tuning, neurons of each T4/T5 subtype orient their dendrites and project their axons in a subtype-specific manner. Directional tuning, thus, appears strictly linked to morphology in T4/T5 neurons. How the four T4/T5 subtypes acquire their distinct morphologies development remains largely unknown. Here, we investigated when and how the dendrites of the four T4/T5 subtypes acquire their specific orientations, and profiled the transcriptomes of all T4/T5 neurons during this process. This revealed a simple and stable combinatorial code of transcription factors defining the four T4/T5 subtypes during their development. Changing the combination of transcription factors of specific T4/T5 subtypes resulted in predictable and complete conversions of subtype-specific properties, i.e. dendrite orientation and matching axon projection pattern. Therefore, a combinatorial code of transcription during factors coordinates the development of dendrite and axon morphologies to generate anatomical specializations differentiating subtypes of T4/T5 motion-sensing neurons.

2020-04-02 | GSE147987 | GEO

Transcription profiling by array of Drosophila embryonic chordotonal neurons expressing the cato GFP reporter gene construct

Project description:Further to a previous transcription profiling experiment using a GFP reporter gene construct expressing the Drosophila proneural gene atonal (Cachero et al. 2011), a later expressing transcription factor and downstream target of ato, cato (cousin of ato) was explored as the GFP reporter in this experiment. cato-GFP expressing cells were isolated from 10h45min and 11h45min old embryos. GFP+ and GFP- cells were compared on Affymetrix Drosophila version 2.0 chips.

2019-02-19 | E-MTAB-7529 | biostudies-arrayexpress

The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons

2011-02-18 | GSE21520 | GEO

Chromatine immunoprecipation of atonal binding sites in Drosophila L3 eye imaginal discs

Project description:Atonal is a proneural transcription factor expressed in the Drosophila eye imaginal discs. To characterize the putative targes of Atonal in the eye discs we have used an endogenously GFP-tagged version of atonal to immunoprecipate disc samples with anti-GFP antibodies

2019-10-09 | GSE138553 | GEO

Modular transcriptional programs separately define axon and dendrite connectivity

Project description:Patterns of synaptic connectivity are remarkably precise and complex. Single-cell RNA sequencing has revealed a vast transcriptional diversity of neurons. Nevertheless, a clear logic underlying the transcriptional control of neuronal connectivity has yet to emerge. Here, we focused on Drosophila T4/T5 neurons, a class of closely related neuronal subtypes with different wiring patterns. Eight subtypes of T4/T5 neurons are defined by combinations of two patterns of dendritic inputs and four patterns of axonal outputs. Single-cell profiling during development revealed distinct transcriptional programs defining each dendrite and axon wiring pattern. These programs were defined by the expression of a few transcription factors and different combinations of cell surface proteins. Gain and loss of function studies provide evidence for independent control of different wiring features. We propose that modular transcriptional programs for distinct wiring features are assembled in different combinations to generate diverse patterns of neuronal connectivity.

2019-11-05 | GSE126139 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data