Genomics

Dataset Information

99

Reprogramming of chromatin accessibility in somatic cell nuclear transfer is DNA replication independent


ABSTRACT: Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNaseI hypersensitive sites (DHSs) in donor cumulus cells and 1-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape of the donor cells is drastically changed to recapitulate that of the in vitro fertilization (IVF)-derived zygotes within 12 hours. Interestingly, this DHS reprogramming takes place even in the presence of a DNA replication inhibitor, suggesting that SCNT-mediated DHS reprogramming is independent of DNA replication. Thus, the study not only reveals the rapid and drastic nature of the changes in chromatin accessibility through SCNT, but also provides a DNA replication-independent model for studying cellular reprogramming. Overall design: Here we performed low-input DNase-Seq (liDNase-Seq) profilling of Donor cumulus cells, 1-Cell stage somantic nuclear Transfer (SCNT) embryos and in 1-Cell Aphidicolin treated SCNT embryos

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Mohamed Nadhir Djekidel  

PROVIDER: GSE110851 | GEO | 2018-05-14

REPOSITORIES: GEO

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Publications

Reprogramming of Chromatin Accessibility in Somatic Cell Nuclear Transfer Is DNA Replication Independent.

Djekidel Mohamed Nadhir MN   Inoue Azusa A   Matoba Shogo S   Suzuki Tsukasa T   Zhang Chunxia C   Lu Falong F   Jiang Lan L   Zhang Yi Y  

Cell reports 20180501 7


Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNase I hypersensitive sites (DHSs) in donor cumulus cells and one-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape  ...[more]

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