Trascriptome profiling of murine Embryonic Stem cells under different culture conditions

ABSTRACT: Mouse Embryonic Stem (ES) cells have been cultured under chemically defined conditions in the absence of feeder cells on Gelatin coated dishes, for 5 passages under 4 different combinations of LIF and GSK3 or MEK inhibitors. Their trascriptome have been analysed by RNA sequencing. The ES cell line used, RexGFPd2, contains a destablised GFP protein in the Zfp42/Rex1 locus and was derived under 2i conditions, as described in Kalkan et al. 2017.

ORGANISM(S): Mus musculus

PROVIDER: GSE111694 | GEO | 2018/11/21

REPOSITORIES: GEO