Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiling of lncRNAs/mRNA for H.pylori infected normal gastric epithelial cell line

ABSTRACT: In this study, cultured H.pylori was used to infect the normal gastric epithelial cell line GES-1, to construct the H.pylori infection model. lncRNA microarray was applied for detecting the lncRNA in H.pylori infection model.

ORGANISM(S): Homo sapiens

PROVIDER: GSE111763 | GEO | 2018/03/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing of GES-1 cells co-cultured with H.pylori with high and low Trx1 expression

Project description:GES-1 gastric epithelial cells were used for co-culturing with H.pylori with high and low Trx1 expression, and RNA sequencing was performed on the co-cultured cells to explore transcriptome differences between the two groups, so as to further explore the pathogenic mechanism of Trx1 in H.pylori.

2022-08-31 | GSE186902 | GEO

LncRNA and mRNA expression profiles from human GES-1 cells in response to Helicobacter pylori infection

Project description:Research on lncRNAs expression profiles in human interactions with Helicobacter pylori (H.pylori) is rare reported. We used HTA2.0 to investigate the expression changes of lncRNAs and mRNAs in human gastric epithelial cells (GES-1 cell line) infected with or without H.pylori infection. Our study provided a preliminary exploration of lncRNAs expression profiles in H.pylori-infected cell models by microarray. A subset of aberrantly expressed lncRNAs was verified by qRT-PCR. These dysregulated lncRNAs might contribute to the pathological processes during H.pylori infection. We infected GES-1 cells with H.pylori as experimental groups,and cells without H.pylori infection were regarded as control groups. After 24hr-infection, cells of each group were selected for total RNA extraction and hybridization on Affymetrix HTA2.0 arrays. The aberrant expression profiles of lncRNAs and mRNAs were explored by microarray analysis between experimental and control groups.

2015-11-03 | E-GEOD-74577 | biostudies-arrayexpress

Air Liquid Interface Model of Gastric Mucosa and H.pylori Infection

Project description:The Air Liquid Interface Model of Gastric Mucosa allows to provide a fully polarized epithelium accessible to infection experiments with H.pylori. The influence of several factors on immune response has been studied.

2018-02-15 | GSE94032 | GEO

LncRNA and mRNA expression profiles from human GES-1 cells in response to Helicobacter pylori infection

2015-11-03 | GSE74577 | GEO

Transcriptome expression changes in human gastric epithelial cells co-cultured with Helicobacter pylori lysate of different generations.

Project description:With the colonization of Helicobacter Pylori (H.pylori) in the human stomach, a large amount of Helicobacter Pylori (H.pylori)colonized into the stomach disintegrates and releases a large number plenty of toxic proteins and other bacterial structure components. TollToll-like receptors (TLRs) in gastric epithelial cells which sensitive to the components of bacteria lysate around the microenvironment attach them and activite innate immune and pro-inflammatory response once contact with antigen. The consequences after long-term stimulation of H.pylori lysate in gastric epithelial cells has not been reported . To investigate the effects of H.pylori lysate on TLRs expression and inflammatory cytokines level during long-term stimulation of gastric epithelial cells and clarify the potential mechanismHence, we detected TLRs mRNA level and inflammatory cytokines expression in GES-1 cells co-culture with lysate from 1 to 30 generations. The sensitivity to H.pylori or H.pylori lysate in GES-1 cells were also monitored. We also explored whether LPS was the main factors contributing to this change. Mongolian gerbils were used to replicate H.pylori infection animal model. After transcriptome sequencing，A TLR2/6 heterodimers agonist Pam2CSK4 and a highly selective inhibitor of PI3K LY294002 were used to clarify the TLR6 signaling pathways. In a conclusion, we discovered that the sensitivity of TLRs in human gastric epithelial cells decreased while after long-time co-culture with H.pylori lysate. Human gastric epithelial cells shows tolerance to H.pylori lysate in TLRs and inflammatory cytokines level. decreased the high level of TLRs and inflammatory cytokines after stimulating GES-1 cells with H.pylori lysate for a short-term. And Tthis process may works through the TLR6/PI3K/NF-κKB axis.

2021-04-18 | GSE149092 | GEO

Expression profiling of lncRNAs/mRNA for 16 human samples derived from patients with altrophic gastritis(Hp+) or gastric cancer(Hp+)

Project description:H.pylori was a well known risk factor for gastic cancer. In this study, we used high-throughtput lncRNA microarray to screen dysregulated lncRNA in gastric cancer, and to investigate the biological function fully understood lncRNA.

2018-03-14 | GSE111762 | GEO

The H.pylori CagA oncoprotein induces replication fork collapse and DNA double strand breaks through Fanconi Anemia pathway downregulation

Project description:Although H.pylori infection is known to induce host genomic double strand breaks, (DSBs), the role of CagA, a central virulence factor with oncogenic properties in DSB generation remains unclear. Here, using transcriptomic and proteomic analyses, we show that CagA downregulates the expression of major DNA repair factors such as FANCD2, BRCA1 and BRCA2 of the Fanconi Anemia (FA) pathway of DNA repair and leads to compromised host replication fork speed, stability and toxic DSB induction. Our results highlight the mechanism of CagA-mediated genome instability at the level of replication forks and can be used to devise strategies that mitigate the genotoxic effects of H.pylori-infection.

2022-03-24 | GSE132356 | GEO

Transcriptome profiling of m6A mRNA modification in GES-1 cells infected by Helicobacter pylori

Project description:Helicobacter pylori (H.pylori) infection is an important factor in the occurrence of human gastric diseases, but its pathogenic mechanism is not clear. N6-methyladenosine (m6A) is the most prevalent reversible methylation modification in mammalian RNA and it plays a crucial role in controlling many biological processes. We used MeRIP-seq technology to sequence the GES-1 cells infected with Helicobacter pylori(H. pylori) for 48 h.

2023-05-11 | GSE230869 | GEO

The proteomic change of GES1 infected by Helicobacter pylori OMVs

Project description:We investigated the effect of H.pylori OMVs on cellular events by detecting proteomic changes in GES1 cells. GES1 cells cocultured with increasing concentrations of H.pylori OMVs were subjected to quantitative proteomic analyses using label-free methods for relative quantitation.

2022-02-14 | PXD025259 | Pride

Functional and molecular dissection of viral lncRNAs throughout HCMV life cycle

Project description:Small, compact genomes confer a selective advantage to viruses, yet human cytomegalovirus (HCMV) expresses the long non-coding RNAs (lncRNAs) RNA1.2, RNA2.7, RNA4.9, and RNA5.0. These lncRNAs account for majority of the viral transcriptome, but their functions remain largely unknown. Here, we showed that HCMV lncRNAs, except for RNA5.0, are required throughout the entire viral life cycle. Deletion of each lncRNA resulted in a decrease in viral progeny during lytic replication and failing to efficiently establish latent reservoirs and reactivate. Nanopore direct RNA sequencing of native lncRNA molecules revealed that each lncRNA exhibited a dynamic modification landscape, depending on the state of infection. Global analysis of the lncRNA interactome identified 32, 11, and 89 host factors that specifically bind to RNA1.2, RNA2.7, and RNA4.9, respectively. Moreover, 52 proteins commonly bound to the three lncRNAs were identified, including 11 antiviral immunity-related proteins. Our molecular analyses found that three lncRNAs are modified with N⁶-methyladenosine (m6A) and interact with m6A readers in all infection states. In-depth functional analysis revealed that m6A–mediated lncRNA stabilization as the key mechanism by which lncRNAs are maintained at high levels. Our study lays the groundwork for understanding viral lncRNA–mediated regulation of host-virus interaction throughout the HCMV life cycle.

2022-11-14 | PXD034587 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data