NMD-degradome sequencing reveals ribosome-bound intermediates with 3'-end nontemplated nucleotides

ABSTRACT: Transcriptome-wide methodology is developed to capture and sequence decay intermediates deriving from direct NMD targets. Results demonstrate that mammalian-cell NMD generates decay intermediates in a process that involves the covalent addition of nontemplated U, C, G and A nucleotides to 3'-ends. Variations of this methodology demonstrate that these decay intermediates arise co-translationally.

ORGANISM(S): Homo sapiens

PROVIDER: GSE111818 | GEO | 2018/07/05

REPOSITORIES: GEO