Genomics

Dataset Information

0

RNAi mediates allele-specific epigenetic inheritance of heterochromatin [ChIP-seq]


ABSTRACT: We tested the ability of trans siRNAs to establish a silent allele of endogenous ade6+ or an ade6+ transgene inserted within euchromatic loci. We found that a silent ade6-OFF epi-allele that grows red on low adenine medium could be established in several mutant backgrounds (mlo3∆, leo1∆) in the presence of ectopic siRNAs. Furthermore, we found that the silent ade6-OFF epi-allele could be meiotically inherited to wildtype progeny lacking the establishing mutation or ectopic siRNAs. Here, we sequenced genome-wide DNA associated with conserved heterochromatin markers and found that silencing of the endogenous ade6 locus correlates with increased spreading of H3K9me2 and H3K9me3. Additionally, heterochromatin domains established with cen::ade6 as the ectopic siRNA-producing locus were associated with significantly more spreading of H3K9me2 and H3K9me3 than those established using an ade6 hairpin as the driver siRNA-producing locus.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE111851 | GEO | 2018/04/26

REPOSITORIES: GEO

Similar Datasets

2018-04-26 | GSE111858 | GEO
| E-GEOD-40674 | biostudies-arrayexpress
2013-12-04 | GSE40674 | GEO
2013-12-26 | GSE52534 | GEO
| E-GEOD-52534 | biostudies-arrayexpress
2021-10-12 | GSE174641 | GEO
| E-GEOD-63304 | biostudies-arrayexpress
2021-07-01 | GSE133741 | GEO
2009-08-01 | GSE16742 | GEO
2017-07-27 | GSE83495 | GEO