Transcriptomics,Genomics

Dataset Information

52

Single cell transcriptomic analysis of human CD25+ CD127- CD4+ Treg cells and CD25- CD127+ CD4+ Tconv cells isolated from peripheral blood


ABSTRACT: Single cell transcriptomic analysis of human CD25+ CD127- CD4+ Treg cells and CD25- CD127+ CD4+ Tconv cells isolated from peripheral blood from two different donors Overall design: Single cells were sorted by flow cytometry for single cell RNAseq library preparation (InDrops)

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

SUBMITTER: CBDM Lab  

PROVIDER: GSE111901 | GEO | 2018-03-15

REPOSITORIES: GEO

altmetric image

Publications

Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR.

Zemmour David D   Zilionis Rapolas R   Kiner Evgeny E   Klein Allon M AM   Mathis Diane D   Benoist Christophe C  

Nature immunology 20180212 3


CD4+ T regulatory cells (Treg) are central to immune homeostasis, their phenotypic heterogeneity reflecting the diverse environments and target cells that they regulate. To understand this heterogeneity, we combined single-cell RNA-seq, activation reporter and T cell receptor (TCR) analysis to profile thousands of Treg or conventional CD4+FoxP3- T cells (Tconv) from mouse lymphoid organs and human blood. Treg and Tconv pools showed areas of overlap, as resting 'furtive' Tregs with overall simila  ...[more]

Similar Datasets

2015-05-01 | E-GEOD-59786 | ArrayExpress
| PRJNA438503 | ENA
2013-06-06 | E-GEOD-30847 | ArrayExpress
2018-02-14 | GSE110547 | GEO
| GSE110568 | GEO
| GSE110688 | GEO
| GSE113625 | GEO
2008-12-19 | E-GEOD-6813 | ArrayExpress
2010-05-19 | E-GEOD-14234 | ArrayExpress
2009-05-20 | GSE14234 | GEO