Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Osmostress induced changes of chromatin architecture and transcription in mammalian cells [ChIP-Seq]

ABSTRACT: Osmostress causes chromatin compaction and simultaneously triggers a massive transcriptional response in eukaryotic cells that is required for adaptation and cell survival. However, thus far it was unknown how changes of chromatin organization and ongoing DNA transcription are linked under hyper-osmotic conditions. In this study, we focused on elucidating the extent of chromatin reoganization upon hyper-osmotic shock, its reversibility and its link with active transcription in human T47D cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE111923 | GEO | 2018/11/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Osmostress induced changes of chromatin architecture and transcription in mammalian cells [RNA-Seq, HiC]

Project description:Osmostress causes chromatin compaction and simultaneously triggers a massive transcriptional response in eukaryotic cells that is required for adaptation and cell survival. However, thus far it was unknown how changes of chromatin organization and ongoing DNA transcription are linked under hyper-osmotic conditions. In this study, we focused on elucidating the extend of chromatin reoganization upon hyper-osmotic shock, its reversibility and its link with active transcription in human T47D cells.

2018-11-23 | GSE111904 | GEO

The yeast osmostress response is carbon source dependent

Project description:Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in particular detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, controlled at different levels by the High Osmolarity Glycerol (HOG) response pathway. Up to now, essentially all osmostress studies in yeast have been performed with glucose as carbon and energy source, which is metabolised by glycolysis with glycerol is as a normal by-product. Here we investigated the response of yeast to osmotic stress when yeast is respiring ethanol as carbon and energy source. Remarkably, yeast cells do not accumulate glycerol under these conditions and it appears that trehalose may partly take over the role as compatible solute. The HOG pathway is activated in very much the same way as in during growth on glucose medium and is also required for osmotic adaptation. Slower volume recovery was observed in ethanol-grown cells as compared to glucose-grown cells. Dependence on key regulators as well as the global gene expression profile were similar in many ways to those previously observed in glucose-grown cells. However, there are indications that cells re-arrange redox-metabolism when respiration is hampered under osmostress, a feature that could not be observed in glucose-grown cells.

2017-02-27 | E-MTAB-5313 | biostudies-arrayexpress

Osmostress induced changes of chromatin architecture and transcription in mammalian cells

Project description:Osmostress induced changes of chromatin architecture and transcription in mammalian cells

| PRJNA438550 | ENA

Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast

Project description:The meiotic cohesin Rec8 is required for the stepwise segregation of chromosomes during the two rounds of meiotic division. By directly measuring chromosome compaction in living cells of the fission yeast Schizosaccharomyces pombe, we found an additional role for the meiotic cohesin in the compaction of chromosomes during meiotic prophase. In the absence of Rec8, chromosomes were decompacted relative to those of wild-type cells. Conversely, loss of the cohesin-associated protein Pds5 resulted in hyper-compaction. While this hyper-compaction requires Rec8, binding of Rec8 to chromatin was reduced in the absence of Pds5, indicating that Pds5 promotes chromosome association of Rec8. To explain these observations, we propose that meiotic prophase chromosomes are organized as chromatin loops emanating from a Rec8-containing axis; the absence of Rec8 disrupts the axis, resulting in disorganized chromosomes, whereas reduced Rec8 loading results in a longitudinally compacted axis with fewer attachment points and longer chromatin loops. Keywords: ChIP-chip analysis ChIP analysis of Rec8: In all cases, hybridization data for ChIP fraction was compared with that of SUP (supernatant) fraction. Pombe chromosome II, III array was used.

2010-07-01 | E-GEOD-5284 | biostudies-arrayexpress

The yeast osmostress response is carbon source dependent

Project description:The yeast osmostress response is carbon source dependent

| PRJEB18911 | ENA

Osmostress induced changes of chromatin architecture and transcription in mammalian cells [ChIP-Seq]

Project description:Osmostress induced changes of chromatin architecture and transcription in mammalian cells [ChIP-Seq]

| PRJNA438548 | ENA

p38 SAPK and SKIIP induced changes in alternative splicing patterns upon osmostress

Project description:Alternative splicing is a crucial mechanism for gene regulation that is modulated in response to a wide range of extracellular stimuli. Stress-activated protein kinases (SAPKs) play a key role in controlling several steps of mRNA biogenesis. Here, we show that osmostress has a major impact on the regulation of alternative splicing (AS), which is partly mediated through the action of the p38 SAPK. Remarkably, a splicing network analysis revealed a functional connection between p38 and the spliceosome component SKIIP. Depletion of SKIIP abolished a significant part of the p38-mediated alternative splicing.

2019-04-16 | GSE117699 | GEO

Genome-wide profiling of histone H1 variants in T47D-MTVL cells

Project description:Human somatic cells may contain up to seven members of the histone H1 family contributing to chromatin compaction and regulation of nuclear processes, apparently with certain subtype specificities. Previous studies in T47D breast cancer cells determined that H1 variants are distributed in a variant-specific manner throughout the genome, although only H1.2 and H1X endogenous variants were mapped. Now, we performed ChIP-seq of five endogenous H1 variants (H1.0, H1.2, H1.4, H1.5, H1X) in T47D cells.

2021-08-16 | GSE166645 | GEO

Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast

2006-08-23 | GSE5284 | GEO

Osmostress induced changes of chromatin architecture and transcription in mammalian cells [RNA-Seq, HiC]

Project description:Osmostress induced changes of chromatin architecture and transcription in mammalian cells [RNA-Seq, HiC]

| PRJNA438511 | ENA