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Transcriptional profile of human STAT1-/- fibroblasts expressing LY6E or empty control vector

ABSTRACT: Detection of viral infection by pattern-recognition receptors triggers production of interferon. Secreted interferon binds to cognate receptors, triggering JAK/STAT signaling, resulting in the transcription and production of hundreds of interferon-stimulated genes (ISGs). Our lab identified lymphocyte antigen 6, locus E (LY6E) as an ISG that enhances infectivity of a subset of enveloped RNA viruses from Flaviviridae, Orthomyxoviridae, and Togaviridae families. To test whether the enhancing effects of LY6E were due to alterations of the global cellular transcriptome, we overexpressed LY6E and a control empty vector and examined global transcription using RNA-Seq.

ORGANISM(S): Homo sapiens

PROVIDER: GSE111958 | GEO | 2018/12/25

REPOSITORIES: GEO

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Transcriptional profile of human STAT1-/- fibroblasts expressing IFI6 or empty control vector

Project description:Detection of viral infection by pattern-recognition receptors triggers production of interferon. Secreted interferon binds to cognate receptors, triggering JAK/STAT signaling, resulting in the transcription and production of hundreds of interferon-stimulated genes (ISGs). Our lab identified interferon alpha inducible protein 6 (IFI6) as an ISG that potently suppresses replication of viruses from the Flavivirus genus. To test whether the inhibitory effects of IFI6 were due to activating expression of other antiviral ISGs, we overexpressed IFI6 and a control vector and examined global transcription using RNA-Seq.

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Transcriptional profile of human STAT1-/- fibroblasts expressing LY6E or empty control vector

Project description:Transcriptional profile of human STAT1-/- fibroblasts expressing LY6E or empty control vector

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Gene expression of LY6E-silent THP-1 cells and control THP-1 cells

Project description:Expression profiling of LY6E-silent THP-1 cells (THP-1-shLY6E) and control cells (THP-1-shCtrl). Results provide evidence that LY6E is effectively knocked down in THP-1shLY6E, compared to THP-1-shCtrl, and show the different expressed genes following LY6E silence in THP-1 cells. Total RNA obtained from 2 cell lines.

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Gene expression of LY6E-silent THP-1 cells and control THP-1 cells

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LY6E blocks coronavirus fusion and confers immune control of viral disease

Project description:LY6E blocks coronavirus fusion and confers immune control of viral disease

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Flaviviridae

Project description:Flaviviridae

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Interferon-stimulated gene expression in STAT1 knockout cells

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