Differentially methylated genes in saliva are linked to childhood stress
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE112314 | GEO | 2018/04/18
REPOSITORIES: GEO
Similar Datasets
Project description:Differentially methylated genes in saliva are linked to childhood stress
| PRJNA450617 | ENA
Project description:DNA methylation from Grady Trauma Project Parental and childhood exposure to trauma increases an individual's lifetime risk for psychiatric and stress-related disorders. This study evaluates DNA methylation in saliva from children, with the goal of identifying associations between peripheral DNA methylation and psychiatric symptoms.
2019-10-02 | GSE138279 | GEO
Project description:In order to investigate age-correlated DNA methylation changes in saliva, genome-wide DNA methylation profiling was performed for saliva from 54 males aged 18 to 73 years. The Illumina Infinium 450k Human DNA methylation Beadchip was used to acquire DNA methylation profiles across about 450,000 CpGs in bisulfite converted DNA.
2017-04-02 | GSE92767 | GEO
Project description:Genome-wide DNA methylation profiling of human blood and saliva samples. The Illumina Infinium HumanMethylation 450k BeadChip was used to obtain DNA methylation profiles across approximately 480,000 CpGs in blood and saliva samples provided from 22 persons, and saliva samples from 3 persons.
2020-03-31 | GSE130153 | GEO
Project description:Genome wide DNA methylation profiling of saliva DNA from 197 alcohol dependent subjects. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in saliva samples.
2011-12-01 | GSE34035 | GEO
Project description:Genome wide DNA methylation profiling of saliva DNA from 197 alcohol dependent subjects. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in saliva samples. Bisulphite converted DNA were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
2011-12-01 | E-GEOD-34035 | biostudies-arrayexpress
Project description:We profiled the DNA methylation of saliva cell types, to develop a tool for epidemiologic studies. Saliva was collected from 22 children, 21 participants with samples usable for DNA methylation, and sorted into immune and epithelial cells, using size exclusion filtration and magnetic bead sorting. DNA methylation was measured using the Illumina MethylationEPIC BeadChip. Saliva immune and epithelial cells have distinct DNA methylation profiles, which can influence whole saliva epidemiologic measures.
2020-09-01 | GSE147318 | GEO
Project description:DNA methylation has become increasingly recognized in the etiology of psychiatric disorders. Because brain tissue is not accessible in living humans, epigenetic studies are most often conducted in blood. Saliva is often collected for genotyping studies but is rarely used to examine DNA methylation because the proportion of epithelial cells and leukocytes varies extensively between individuals. The goal of this study was to evaluate whether saliva DNA is informative for studies of psychiatric disorders. Saliva and blood methylation was clearly distinguishable though there was positive correlation overall. There was little correlation in CpG sites within relevant candidate genes. Correlated CpG sites were more likely to occur in areas of low CpG density (i.e. CpG shores and open seas). There was more variability in CpG sites from saliva than blood, which may reflect its heterogeneity. Thus, this study provides a framework for using DNA methylation from saliva and suggests that DNA methylation of saliva may offer distinct opportunities for epidemiological and longitudinal studies of psychiatric traits. DNA methylation was assessed in saliva and blood samples from 64 adult African Americans. Both saliva and blood samples were collected from each participant. Saliva was stored in Oragene DNA sample collection kits (DNA Genotek), and blood was collected in EDTA vacuum tubes. DNA was extracted using the Puregene Genomic DNA kit (Invitrogen). DNA methylation was interrogated for each sample using the HumanMethylation450 BeadChip (Illumina). Analyses for tissue-specific DNA patterns were conducted using linear regression adjusted for appropriate covariates, including estimated cellular proportions. The estimated proportion of epithelial cells in saliva DNA ranged from 3-99% (median 26%). In blood, the estimated proportion of lymphocytes ranged from 25-70% (median 48%) and neutrophils ranged from 42-84% (median 58%). Methylation of 68.8% of all CpG sites differed relative to epithelial cell proportion (FDR<.05). Our results provide a framework for methylation studies in DNA extracted from saliva and highlight the importance of controlling for the proportion of epithelial and leukocyte cells. This presents an attractive opportunity for investigators that have already collected salivary DNA for genetic studies of psychiatric traits.
2014-09-24 | E-GEOD-61653 | biostudies-arrayexpress
Project description:DNA methylation has become increasingly recognized in the etiology of psychiatric disorders. Because brain tissue is not accessible in living humans, epigenetic studies are most often conducted in blood. Saliva is often collected for genotyping studies but is rarely used to examine DNA methylation because the proportion of epithelial cells and leukocytes varies extensively between individuals. The goal of this study was to evaluate whether saliva DNA is informative for studies of psychiatric disorders. Saliva and blood methylation was clearly distinguishable though there was positive correlation overall. There was little correlation in CpG sites within relevant candidate genes. Correlated CpG sites were more likely to occur in areas of low CpG density (i.e. CpG shores and open seas). There was more variability in CpG sites from saliva than blood, which may reflect its heterogeneity. Thus, this study provides a framework for using DNA methylation from saliva and suggests that DNA methylation of saliva may offer distinct opportunities for epidemiological and longitudinal studies of psychiatric traits.
2014-09-24 | GSE61653 | GEO
Project description:Human DNA methylation Beadchip v1.2 was used to analyze n=259 human saliva samples from Caucasians (non-Hispanic whites) and Hispanics collected from the PEG study (directed by Dr Beate Ritz). The main goal of the study was to measure the epigenetic age (also known as DNA methylation age) of saliva. To measure DNA methylation age, we used the epigenetic clock software described in Horvath S (n=2013) DNA methylation age of human tissues and cell types. Genome Biology.2013, 14:R115. DOI: 10.1186/10.1186/gb-2013-14-10-r115 PMID: 24138928.
2016-07-13 | GSE78874 | GEO