Genomics

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Genome-wide maps of SMAD2-chromatin binding state in TGFbeta-treated vascular smooth muscle cells


ABSTRACT: To further explore the role of these two factors in SMC reprogramming, we treated human aortic smooth muscle (HASMCs) in vitro with TGFβ and mapped binding of Smad2/3, which reflects TGFβ activity, and Pol2-Ser2p, which reflects transcriptional activity, by ChIP-seq analysis. Smad2/3 bound to numerous regulatory regions in the genome differentially regulating expression of numerous genes as demonstrate by alterations in Pol2-Serp2 binding profile and analysis of bulk RNA sequencing. Examination of the top 20 transcription factors identified by sequencing of Smad2/3 binding regulatory regions identified KLF4 gene as one of the most prominently regulated genes. Further analysis showed Smad2/3 binding to multiple KLF4 gene regulatory elements that increased following TGFβ treatment, indicating that KLF4 is a direct target of Smad2/3. At the same time, the amount of bound Pol2-Ser2p decreased, pointing to reduced transcriptional activity. In contrast, Smad2/3 did not bind to KLF2 or KLF5 regulatory elements, demonstrating that these genes are not directly regulated by TGFβ. Overall design: Examination of the binding of SMAD in chromatin of vascular smooth muscle cell.

INSTRUMENT(S): Illumina HiSeq 4000 (Homo sapiens)

SUBMITTER: zehua chen  

PROVIDER: GSE112326 | GEO | 2018-03-27

REPOSITORIES: GEO

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