Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

ABSTRACT: ChIP-seq of FLAG-tagged RPA190 (subunit of RNA Polymerase I) or FLAG-tagged RPO31 (subunit of RNA Polymerase III) was performed on WT and isw2∆ nhp10∆ cells. Sample naming convention: ___

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE112425 | GEO | 2018/10/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

Project description:ChIP-seq of FLAG-tagged Pol2 (subunit of DNA Polymerase epsilon) was performed on two independent isolates of each of WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112426 | GEO

Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

Project description:ChIP-seq of Isw2 (subunit of Isw2 complex) and Nhp10 (subunit of Ino80 complex) was performed on two independent isolates of FLAG-tagged Isw2 or Nhp10 Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112423 | GEO

Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

Project description:MNase-seq was performed in WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: MNase_<growth condition>_<genotype-replicate>_<strain name>_<units MNase>

2018-10-30 | GSE112427 | GEO

Genome-wide maps of histone H3 in asynchronously growing yeast cells

Project description:ChIP-seq of histone H3 was performed on two biological replicates of the same W303 strain of wild-type cells and two independent isolates of each of isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112424 | GEO

Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation (ChIP-seq)

Project description:SAGA is a highly conserved transcriptional co-activator complex involved in multiple steps of transcription with activities that function both pre and post initiation. Loss of individual subunits results in developmental defects, suggesting a role in development. To better understand the roles of SAGA functions in developmental gene expression and it's relationship with RNA polymerase II, we examined its composition, binding profile, and the effects of subunit loss on gene expression in two distinct cell types in late stage Drosophila embryos: muscle and neurons. Chromatin IP of FLAG-tagged SAGA subunit Ada2b, and RNA polymerase II (antibody 4H8), was performed in neuronal (elav+) or muscle (mef2+) cells isolated by FACS from late stage embryos, and compared to whole cell extracts (input). The control constists of an IP performed in neuronal cells from a non-tagged strain.

2011-08-22 | E-GEOD-29513 | biostudies-arrayexpress

The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo

Project description:We addressed the roles of three nucleosome spacing enzymes (ISW1, ISW2 and CHD1) in specifying chromatin organization in S. cerevisiae. Comparison of budding yeast nucleosome positions obtained using micrococcal nuclease digestion followed by paired-end sequencing (MNase-seq). We sequenced nucleosomes from isogenic strains with the isw1Î, isw2Î or chd1Î mutations in all combinations. We measured gene activity by ChIP-seq for the Rpb3 subunit of RNA polymerase II for some of these mutants.

2016-02-17 | E-GEOD-69400 | biostudies-arrayexpress

FLAG-tagged FOXM1A Plasmid

Project description:FLAG-tagged FOXM1A Plasmid

| PRJEB24606 | ENA

Murine norovirus replication induces G0/G1 cell cycle arrest in asynchronously growing cells.

Project description: Not available

| S-EPMC4442456 | biostudies-literature

Saccharomyces cerevisiae

Project description:Yeast replication in the presence of MMS: WT and isw2 nhp10 strains

| PRJNA102659 | ENA

Histone octamer structure is altered early in ISW2 ATP-dependent remodeling of nucleosomes

Project description:Histone octamers are thought to be a rigid part of nucleosomes that shape chromatin and block cellular machinery from accessing DNA. ATP-dependent chromatin remodelers like ISW2 mobilize nucleosomes to provide DNA access. We find evidence for histone octamer distortion preceding DNA being moved into nucleosomes and processive movement of the ATPase motor of ISW2 on nucleosomal DNA. DNA entering nucleosome is uncoupled from the ATPase activity of ISW2 and alterations of the histone octamer structure mediated by ISW2 by deletion of the SANT domain from the C-terminus of the Isw2 catalytic subunit. We also find that restricting histone movement by chemical crosslinking traps remodeling intermediates resembling those seen by loss of the SANT domain, further supporting the importance of changes in histone octamer structure early in ISW2 remodeling. Transient octamer distortions are stabilized by H3-H4 tetramer disulfide crosslinking, thereby linking intrinsic histone octamer flexibility to chromatin remodeling.

2020-05-27 | PXD013967 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data