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Characterization of the innate immune response to Streptococcus pneumoniae infection in zebrafish

ABSTRACT: we characterized the zebrafish innate immune response to pneumococcus through a whole-genome level transcriptome analysis. The analysis revealed the induction of genes coding for pro-inflammatory cytokines, chemokines, acute phase proteins, and antimicrobial peptides, indicating a well-conserved innate immune response to the human pathogen pneumococcus in zebrafish embryos. To gain understanding of the genetic factors associated with the increased risk for severe pneumococcal infection in humans, we carried out a medium-scale forward genetic screen in zebrafish. We identified a mutant fish line, which showed compromised defense against pneumococcus in septic larval infection model. Poor survival upon infection was associated with increased bacterial counts indicating defect in resistance. The transcriptome analysis of the mutant zebrafish embryos revealed otherwise normal innate immune response to pneumococcal infection but a deficient expression of a gene homologous for human C-reactive protein (CRP).

ORGANISM(S): Danio rerio

PROVIDER: GSE112433 | GEO | 2022/11/14

REPOSITORIES: GEO

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Dual RNA-seq on pneumococcal infection to zebrafish embryo model

Project description:Zebrafish embryo has been emerging as an interesting model of infection due to their fecundity, transparency and availability of genetic tools. Streptococcus pneumoniae, the pneumococcus, is the main etiological agent of pneumonia, sepsis and meningitis. The bacteria expresses a very important virulence factor, pneumolysin able to form pores on cholesterol-based membranes and to activate innate immune system. Here, we exploited the recently described dual RNA-seq to simultaneously measure genome-wide expression of host and pathogen eight hours into infection. Functional enrichment analysis showed certain pathways such as autophagy and apoptosis being activated in the host while stress responses including pneumococcal competence. The study is the first to describe dual RNA-seq application in whole organism sequencing in infection model.

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