Dataset Information


Genome-Wide profile of c-Myc binding in HL60 cells

ABSTRACT: Analysis of technical variance of ChIP-on-Chip studies by characterization of Myc-binding in HL60 cells. Keywords: ChIP, c-Myc Overall design: ChIP-on-chip against c-Myc, each array is one biological replicate

INSTRUMENT(S): Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version)

SUBMITTER: Paul C Boutros  

PROVIDER: GSE11245 | GEO | 2008-11-01



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Optimization of experimental design parameters for high-throughput chromatin immunoprecipitation studies.

Ponzielli Romina R   Boutros Paul C PC   Katz Sigal S   Stojanova Angelina A   Hanley Adam P AP   Khosravi Fereshteh F   Bros Christina C   Jurisica Igor I   Penn Linda Z LZ  

Nucleic acids research 20081021 21

High-throughput, microarray-based chromatin immunoprecipitation (ChIP-chip) technology allows in vivo elucidation of transcriptional networks. However this complex is not yet readily accessible, in part because its many parameters have not been systematically evaluated and optimized. We address this gap by systematically assessing experimental-design parameters including antibody purity, dye-bias, array-batch, inter-day hybridization bias, amplification method and choice of hybridization control  ...[more]

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