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RNAseq of SEC23B wildtype (WT) and mutant (MUT) thyroid cell lines treated with Tunicamycin (Tunica, ER stress)

ABSTRACT: We report gene expression changes between SEC23B WT and MUT cells after treatment with Tunicamycin. The overall goal is to agnostically determine impacted signaling pathways in the wildtype and mutant settings.

ORGANISM(S): Homo sapiens

PROVIDER: GSE112661 | GEO | 2020/08/31

REPOSITORIES: GEO

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RNAseq of SEC23B wildtype (WT) and mutant (MUT) thyroid cell lines treated with Tunicamycin (Tunica, ER stress)

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Project description:To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted. Among them, expression of three miRNAs (miR-23a, miR-27a, miR-24-2) was quantified in the RNA samples from the same as the microarray, and COS7 cells by real-time PCR, confirming existence of similar mechanisms of trancriptional repression in ER stress by tunicamycin treatment. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with 2ug/mL tunicamycin for 12 or 24 hrs. Two independent experiments were performed at each time (untreated, 12 or 24 hrs). miRNAs responsible for tunicamycin-treatment for 12hrs in XBP1-dependent manner were extracted.

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