Genomics

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Small RNA sequences from grafted Arabidopsis thaliana expressing SucSUL inverted repeat


ABSTRACT: We aimed at characterizing the identity of the silencing signal i.e. siRNA or longer dsRNA precursor thereof. We used transgenic dsRNA, endogenous dsRNA or virus infection as sources of siRNAs combined to sophisticated immunoprecipitation-based procedures coupled to deep-sequencing. We found that siRNAs populations are highly similar between incipient and recipient cells in the three systems. Additionally, a significant depletion of 5’U and 5’A content in the recipient cells suggested Argonaute-loading dependent depletion of these siRNAs during their movement over multiple cell layers. Using the PAZ-domain mutants ago1-18 and ago1-42 confirmed that the observed depletion of mobile siRNAs is the result of RISC loading. The results advocate movement of individual siRNA duplexes as opposed to their precursors and that loading into Argonaut proteins renders those small RNAs cell-autonomous. Overall design: 4 samples examined: shoot and root from Arabidopsis thaliana where plant expressing SucSUL transgene were grafted to Col0 WT plants (in both directions)

INSTRUMENT(S): Illumina HiSeq 2500 (Arabidopsis thaliana)

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Alexis Sarazin  

PROVIDER: GSE112885 | GEO | 2020-04-06

REPOSITORIES: GEO

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