Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing (NGS) of expression profile from unstimulated and LPS stimulated bone marrow derived macrophages from control and BRD4 conditional KO mice

ABSTRACT: Method: mRNA profiles were generated from pair-end sequencing of duplicate samples using Illumina Hiseq 2000. Results: Genes with an expression change of more than 2 fold were considered to be differentially expresed

ORGANISM(S): Mus musculus

PROVIDER: GSE113009 | GEO | 2019/02/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Bulk RNA-seq on unstimulated and LPS-stimulated human macrophages

Project description:The goal of this experiment is to profile LPS-stimulated transcriptome changes in human macrophages. Human whole blood was from the Sanofi in-house blood donor service that is approved by the local ethics committee and all blood donors signed informed consent. Human peripheral blood monocytes were isolated from anonymous donors’ prefinal blood using Ficoll density centrifugation, followed by magnetic separation with positive selection (CD14 MicroBeads, Miltenyi Biotec). Monocytes were differentiated into macrophages by culturing in macrophage serum-free medium (Life Technologies) containing 50 ng/ml recombinant human macrophage colony-stimulating factor (Immunotools) for 5 days. Following differentiation, cells were cultured in RPMI 1640 medium supplemented with 10% heat inactivated fetal bovine serum (Thermo Fisher) and maintained at 37°C in a 5% CO2/air environment. Macrophages were stimulated with 50 ng/ml lipopolysaccharides from Escherichia coli O111:B4 (Sigma) for 24 hours.

2022-03-24 | GSE193336 | GEO

Stimulated and unstimulated saliva samples have significantly different bacterial profiles.

Project description: Not available

| S-EPMC5983451 | biostudies-literature

Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples.

Project description: Not available

| S-EPMC4796727 | biostudies-literature

Next Generation Sequencing (NGS) of expression profile from unstimulated and LPS stimulated bone marrow derived macrophages from control and BRD4 conditional KO mice

Project description:Next Generation Sequencing (NGS) of expression profile from unstimulated and LPS stimulated bone marrow derived macrophages from control and BRD4 conditional KO mice

| PRJNA449757 | ENA

Microarray for LPS-stimulated B cells compared to unstimulated B cells

Project description:Both multiple myeloma (MM) and systemic lupus erythematosus (SLE) are characterized with abnormal production of plasma cells. To explore the novel therapeutic target to plasma cells, we determined the gene expression profile in plasma cells by affymetrix microarrays. LPS could effectively in vitro induce the production of plasma cells. The splenocytes were separated from three C57BL/6 mice per group. B cells were sorted by B220 microbeads, and stimulated for 3 days in vitro by 10 ug/ml LPS. The transcripts in LPS-stimulated B cells were determined by Affymetrix Microarrays.

2019-08-01 | E-MTAB-7109 | biostudies-arrayexpress

Cytokine Stimulated Vs Unstimulated

Project description:human bronchial smooth muscle cells were growth arrested. One group was stimulated with 10ng/mL of IL-1beta, TNF-alfa, and gamma IFN for 20 hours. For every cDNA sample, 2 dyeflipped slides were hybridized (these have the same date in the titles). All cy3 (channel 2) measurements were normalized to the dye coupling control before a ratio of the values was taken. Keywords: parallel sample

2004-04-09 | GSE1288 | GEO

Comparison of oral metabolome profiles of stimulated saliva, unstimulated saliva, and mouth-rinsed water.

Project description: Not available

| S-EPMC8758762 | biostudies-literature

Comparison of oral microbiome profiles in stimulated and unstimulated saliva, tongue, and mouth-rinsed water.

Project description: Not available

| S-EPMC6834574 | biostudies-literature

Glucose controls morphodynamics of LPS-stimulated macrophages.

Project description: Not available

| S-EPMC4010488 | biostudies-literature

Bulk RNA-seq on unstimulated and LPS-stimulated human macrophages

Project description:Bulk RNA-seq on unstimulated and LPS-stimulated human macrophages