Dataset Information


Gene expression profiling of tumor-associated macrophages and in-vitro cultured macrophages

ABSTRACT: The aim of this study was to decipher gene expression of tumor-associated macrophage in the 4T1 mouse model of breast cancer. Overall design: For M0 basal macrophages, Ly6C+ monocytes were isolated from splenocytes of 4T1-bearing mice using Miltenyi magnetic beads, seeded in a 6-wells plate at 1 million cells per wells in RPMI 10% FCS, penicillin/streptomycin, 50ng/ml M-CSF and cultured for 72h. F4/80+ TAMs were isolated from enzyme-digested 4T1 tumors using Miltenyi magnetic beads, and lysed directly in Ambion's PureLink™ RNA Mini Kit lysis buffer.

INSTRUMENT(S): Illumina MouseWG-6 v2.0 R2 expression beadchip [gene symbol version]


PROVIDER: GSE113034 | GEO | 2018-06-01


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