Transcriptomics,Genomics

Dataset Information

0

Gene expression profiling of tumor-associated macrophages and in-vitro cultured macrophages


ABSTRACT: The aim of this study was to decipher gene expression of tumor-associated macrophage in the 4T1 mouse model of breast cancer. Overall design: For M0 basal macrophages, Ly6C+ monocytes were isolated from splenocytes of 4T1-bearing mice using Miltenyi magnetic beads, seeded in a 6-wells plate at 1 million cells per wells in RPMI 10% FCS, penicillin/streptomycin, 50ng/ml M-CSF and cultured for 72h. F4/80+ TAMs were isolated from enzyme-digested 4T1 tumors using Miltenyi magnetic beads, and lysed directly in Ambion's PureLink™ RNA Mini Kit lysis buffer.

INSTRUMENT(S): Illumina MouseWG-6 v2.0 R2 expression beadchip [gene symbol version]

SUBMITTER: JONATHAN CARON   

PROVIDER: GSE113034 | GEO | 2018-06-01

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2015-05-02 | E-GEOD-59047 | ArrayExpress
| GSE86298 | GEO
2014-06-03 | E-GEOD-49625 | ArrayExpress
2016-02-20 | E-GEOD-57785 | ArrayExpress
| GSE70404 | GEO
2014-06-03 | E-GEOD-46984 | ArrayExpress
2012-10-22 | E-GEOD-41604 | ArrayExpress
2014-05-04 | E-GEOD-34903 | ArrayExpress
2016-07-10 | E-GEOD-74783 | ArrayExpress
2008-02-14 | GSE10500 | GEO