Transcriptomics

Dataset Information

24

Tissue-selective restriction of RNA editing of CaV1.3 by splicing factor SRSF9


ABSTRACT: Adenosine DeAminases acting on RNA (ADAR) catalyzes adenosine-to-inosine (A-to-I) conversion within RNA duplex structures. While A-to-I editing is often dynamically regulated in a spatial-temporal manner, the mechanisms underlying its tissue-selective restriction remain elusive. We have previously reported that transcripts of voltage-gated calcium channel CaV1.3 are subject to brain-selective A-to-I RNA editing by ADAR2. Here, we show that editing of CaV1.3 mRNA is dependent on a 40 bp RNA duplex formed between exon 41 and an evolutionarily conserved editing site complementary sequence (ECS) located within the preceding intron. Heterologous expression of a mouse minigene that contained the ECS, intermediate intronic sequence and exon 41 with ADAR2 yielded robust editing. Interestingly, editing of CaV1.3 was potently inhibited by serine/arginine-rich splicing factor 9 (SRSF9). Mechanistically, the inhibitory effect of SRSF9 required direct RNA interaction. Selective down-regulation of SRSF9 in neurons provides a basis for the neuron-specific editing of CaV1.3 transcripts. Overall design: eCLIP-seq for HA-SRSF9 in HEK293 cells

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Tuck Wah Soong   

PROVIDER: GSE113193 | GEO | 2018-04-17

REPOSITORIES: GEO

altmetric image

Publications


Adenosine DeAminases acting on RNA (ADAR) catalyzes adenosine-to-inosine (A-to-I) conversion within RNA duplex structures. While A-to-I editing is often dynamically regulated in a spatial-temporal manner, the mechanisms underlying its tissue-selective restriction remain elusive. We have previously reported that transcripts of voltage-gated calcium channel CaV1.3 are subject to brain-selective A-to-I RNA editing by ADAR2. Here, we show that editing of CaV1.3 mRNA is dependent on a 40 bp RNA duple  ...[more]

Similar Datasets

2016-12-28 | E-GEOD-70588 | ArrayExpress
2014-10-03 | E-GEOD-61066 | ArrayExpress
2014-10-03 | E-GEOD-61067 | ArrayExpress
2014-05-01 | E-GEOD-15383 | ArrayExpress
2010-11-01 | GSE15383 | GEO
2012-02-15 | E-GEOD-34626 | ArrayExpress
| GSE74746 | GEO
| GSE94387 | GEO
| GSE94388 | GEO
2013-09-30 | E-GEOD-38233 | ArrayExpress