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The transcription factor Rfx7 limits metabolism of NK cells promoting their maintenance and immunity

ABSTRACT: Regulatory factor X 7 (Rfx7) is an uncharacterized member of a family of transcription factors playing essential functions in ciliogenesis and immunity. Given the prominent expression of Rfx7 in lymphoid organs, we generated mice with Rfx7-deletion in the hematopoietic compartment. We assessed the function of Rfx7 in natural killer cells, which are innate lymphoid cells that protect from viral infections and cancer. By comparing the transcriptome of Rfx7-deficient and control NK cells isolated from bone marrow and spleen, we identified negative regulators of cell metabolism among the genes regulated by this transcription factor. Overall design: There are 12 NK cells samples profiles for transcriptomics by RNA sequencing. NK cells come either from spleen or bone marrow, and are either RFX7 wild-type (from Vavicre Rfx7wt/wt mice) or knock-out (from Vavicre Rfx7fl/fl mice) (in triplicate for each condition).

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: David Barras  

PROVIDER: GSE113267 | GEO | 2018-06-26


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Regulatory factor X 7 (Rfx7) is an uncharacterized transcription factor belonging to a family involved in ciliogenesis and immunity. Here, we found that deletion of Rfx7 leads to a decrease in natural killer (NK) cell maintenance and immunity in vivo. Genomic approaches showed that Rfx7 coordinated a transcriptional network controlling cell metabolism. Rfx7<sup>-/-</sup> NK lymphocytes presented increased size, granularity, proliferation, and energetic state, whereas genetic reduction of mTOR ac  ...[more]

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