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MGAS5005 vs MGAS5005∆ccpA

ABSTRACT: The aim of this study was to determine the extent of the CcpA regulon in the M1 serotype MGAS5005. Kinkel and McIver, 2008 Infection and Immunity Keywords: group A streptococcus, CcpA

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE11328 | GEO | 2008/06/04

SECONDARY ACCESSION(S): PRJNA106651

REPOSITORIES: GEO

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MGAS5005 vs MGAS5005∆ccpA

Project description:The aim of this study was to determine the extent of the CcpA regulon in the M1 serotype MGAS5005. Kinkel and McIver, 2008 Infection and Immunity Keywords: group A streptococcus, CcpA 3 biological replicates of MGAS5005 and the isogenic ∆ccpA strain were grown, and RNA was isolated independently 3 times. The RNA was converted to cDNA and labeled with with either Cy3 or Cy5 using the GE Healthcare CyScribe Post-labelling kit. The arrays were scanned with GenePix 6.1 and analyzed with Acuity 4.0.

2008-06-04 | E-GEOD-11328 | biostudies-arrayexpress

M1 MGAS5005 TrxR+ vs. TrxR- mutant

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M1 MGAS5005 TrxR+ vs. TrxR- mutant

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Project description:This study evaluate how catabolite control protein A (CcpA) inactivation affected gene transcript levels in the group A Streptococcus serotype M1 strain MGAS5005 during growth in standard laboratory medium. Keywords: transcriptome analysis 4 replicates of strain MGAS5005 and delta_cppA were grown in THY to early and late logarithmic growth phases. The transcriptomes of each samples were then analyzed.

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Project description:We sought to determine how deletion of ccpA affects the gene expression profile in three M serotype strains of group A streptococcus

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ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Galactose at MID-log growth phase

Project description:Comparison of Streptococcus pneumoniae D39 ccpA mutant compared to D39 wild type in CDM with galactose as sole carbon source to define the regulon of carbon catabolite control protein CcpA under this condition Details described in Carvalho SM, Kloosterman TG, Neves AR, Kuipers OP. CcpA Ensures Optimal Metabolic Fitness of Streptococcus pneumoniae. Plos One 2011

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ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Glucose at MID-log growth phase

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ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + galactose at transition-phase of growth (TS)

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